Bioinformatics Analysis of Potential Key Genes in Trastuzumab-Resistant Gastric Cancer
Joint Authors
Yang, Guangda
Jian, Liumeng
Lin, Xiangan
Zhu, Aiyu
Wen, Guohua
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
This study was performed to identify genes related to acquired trastuzumab resistance in gastric cancer (GC) and to analyze their prognostic value.
Methods.
The gene expression profile GSE77346 was downloaded from the Gene Expression Omnibus (GEO) database.
Differentially expressed genes (DEGs) were obtained by using GEO2R.
Functional and pathway enrichment was analyzed by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).
Search Tool for the Retrieval of Interacting Genes (STRING), Cytoscape, and MCODE were then used to construct the protein-protein interaction (PPI) network and identify hub genes.
Finally, the relationship between hub genes and overall survival (OS) was analyzed by using the online Kaplan-Meier plotter tool.
Results.
A total of 327 DEGs were screened and were mainly enriched in terms related to pathways in cancer, signaling pathways regulating stem cell pluripotency, HTLV-I infection, and ECM-receptor interactions.
A PPI network was constructed, and 18 hub genes (including one upregulated gene and seventeen downregulated genes) were identified based on the degrees and MCODE scores of the PPI network.
Finally, the expression of four hub genes (ERBB2, VIM, EGR1, and PSMB8) was found to be related to the prognosis of HER2-positive (HER2+) gastric cancer.
However, the prognostic value of the other hub genes was controversial; interestingly, most of these genes were interferon- (IFN-) stimulated genes (ISGs).
Conclusions.
Overall, we propose that the four hub genes may be potential targets in trastuzumab-resistant gastric cancer and that ISGs may play a key role in promoting trastuzumab resistance in GC.
American Psychological Association (APA)
Yang, Guangda& Jian, Liumeng& Lin, Xiangan& Zhu, Aiyu& Wen, Guohua. 2019. Bioinformatics Analysis of Potential Key Genes in Trastuzumab-Resistant Gastric Cancer. Disease Markers،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1146869
Modern Language Association (MLA)
Yang, Guangda…[et al.]. Bioinformatics Analysis of Potential Key Genes in Trastuzumab-Resistant Gastric Cancer. Disease Markers No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1146869
American Medical Association (AMA)
Yang, Guangda& Jian, Liumeng& Lin, Xiangan& Zhu, Aiyu& Wen, Guohua. Bioinformatics Analysis of Potential Key Genes in Trastuzumab-Resistant Gastric Cancer. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1146869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1146869