DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATRp53Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma
Joint Authors
Yang, Jiechao
Zhou, Liang
Zhang, Yanping
Zheng, Juan
Zhou, Jian
Wei, Zheqiang
Zou, Jiaping
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cancer bioinformatics has been used to screen possible key cancer genes and pathways.
Here, through bioinformatics analysis, we found that high expression of diaphanous related formin 1 (DIAPH1) was associated with poor overall survival in head and neck squamous cell carcinoma and laryngeal squamous cell carcinoma (LSCC).
The effect of DIAPH1 in LSCC has not been previously investigated.
Therefore, we evaluated the expression, function, and molecular mechanisms of DIAPH1 in LSCC.
Immunohistochemistry and western blot analysis confirmed the significant upregulation of DIAPH1 in LSCC.
We used DIAPH1 RNA interference to construct two DIAPH1-knockdown LSCC cell lines, AMC-HN-8 and FD-LSC-1, and validated the knockdown efficiency.
Flow cytometry data showed that DIAPH1 inhibited apoptosis.
Further, western blot analysis revealed that DIAPH1 knockdown increased the protein levels of ATR, p-p53, Bax, and cleaved caspase-3, -8, and -9.
Thus, DIAPH1 is upregulated in LSCC and may act as an oncogene by inhibiting apoptosis through the ATR/p53/caspase-3 pathway in LSCC cells.
American Psychological Association (APA)
Yang, Jiechao& Zhou, Liang& Zhang, Yanping& Zheng, Juan& Zhou, Jian& Wei, Zheqiang…[et al.]. 2019. DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATRp53Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma. Disease Markers،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1147606
Modern Language Association (MLA)
Yang, Jiechao…[et al.]. DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATRp53Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma. Disease Markers No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1147606
American Medical Association (AMA)
Yang, Jiechao& Zhou, Liang& Zhang, Yanping& Zheng, Juan& Zhou, Jian& Wei, Zheqiang…[et al.]. DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATRp53Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1147606
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1147606