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Placental Expression of NEMO Protein in Normal Pregnancy and Preeclampsia
Joint Authors
Sakowicz, Agata
Lisowska, Michalina
Biesiada, Lidia
Płuciennik, Elżbieta
Gach, Agnieszka
Rybak-Krzyszkowska, Magda
Huras, Hubert
Sakowicz, Bartosz
Romanowicz, Hanna
Piastowska-Ciesielska, Agnieszka W.
Grzesiak, Mariusz
Pietrucha, Tadeusz
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Preeclamptic pregnancies often present an intensified inflammatory state associated with the nuclear activity of NFκB.
NEMO is an essential regulator of nuclear factor kappa B (NFκB) in cytoplasmic and nuclear cellular compartments.
The aim of the present study is to examine the level and localization of the NEMO protein in preeclamptic and nonpreeclamptic placentas.
Methods.
The study includes 97 preeclamptic cases and 88 controls.
NEMO distribution was analyzed immunohistochemically.
Its localization in the nuclear and cytoplasmic fractions, as well as in total homogenates of placental samples, was studied by western blot and ELISA.
Results.
The western blot and ELISA results indicate a significant difference in NEMO concentration in the total and nuclear fractions between preeclamptic and control samples (p<0.01 and p<0.001, respectively).
In the cytoplasmic complement, similar levels of NEMO were found in preeclamptic and control placentas.
In addition, immunohistochemical staining revealed that the NEMO protein is mainly localized in the syncytiotrophoblast layer, with controls demonstrating a stronger reaction with NEMO antibodies.
This study also shows that the placental level of NEMO depends on the sex of the fetus.
Conclusions.
The depletion of the NEMO protein in the cellular compartments of placental samples may activate one of the molecular pathways influencing the development of preeclampsia, especially in pregnancies with a female fetus.
A reduction of the NEMO protein in the nuclear fraction of preeclamptic placentas may intensify the inflammatory state characteristic for preeclampsia and increase the level of apoptosis and necrosis within preeclamptic placentas.
American Psychological Association (APA)
Sakowicz, Agata& Lisowska, Michalina& Biesiada, Lidia& Płuciennik, Elżbieta& Gach, Agnieszka& Rybak-Krzyszkowska, Magda…[et al.]. 2019. Placental Expression of NEMO Protein in Normal Pregnancy and Preeclampsia. Disease Markers،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1147866
Modern Language Association (MLA)
Sakowicz, Agata…[et al.]. Placental Expression of NEMO Protein in Normal Pregnancy and Preeclampsia. Disease Markers No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1147866
American Medical Association (AMA)
Sakowicz, Agata& Lisowska, Michalina& Biesiada, Lidia& Płuciennik, Elżbieta& Gach, Agnieszka& Rybak-Krzyszkowska, Magda…[et al.]. Placental Expression of NEMO Protein in Normal Pregnancy and Preeclampsia. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1147866
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1147866