![](/images/graphics-bg.png)
Bufei Jianpi Granules Reduce Quadriceps Muscular Cell Apoptosis by Improving Mitochondrial Function in Rats with Chronic Obstructive Pulmonary Disease
Joint Authors
Jian-sheng, Li
Ya, Li
Tian, Yange
Li, Suyun
Mao, Jing
Feng, Suxiang
Liu, Xuefang
Bian, Qingqing
Li, Junzi
Hu, Yuanyuan
Zhang, Lanxi
Ji, Huige
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Cell apoptosis is an important mechanism underlying skeletal muscle dysfunction in chronic obstructive pulmonary disease (COPD) patients, and mitochondrial dysfunction is recognized as a central aspect contributing to skeletal muscle deterioration.
Bufei Jianpi granules have been confirmed effective for improving motor function in COPD patients, but the specific mechanism for this improved function remains unknown.
This study explored the mechanisms by which Bufei Jianpi granules improve cell apoptosis and mitochondrial dysfunction in COPD.
Methods.
Sprague-Dawley rats were randomized into control, model, Bufei Jianpi, and aminophylline groups.
A stable COPD rat model was induced with respective repeated cigarette smoke inhalation and intragastric bacterial infection, and rats were sacrificed after 20 weeks; the quadriceps muscle was harvested from each rat.
Skeletal muscle mitochondria were extracted for measurements of mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore openings (mPTPs).
ATP levels were determined with a firefly luciferase-based ATP assay kit.
The rates of cell apoptosis were determined by the transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) method.
Cyto C and caspase-3 mRNA and protein levels were measured by qPCR and western blotting.
Results.
ATP, MMP, and mPTPs were markedly decreased in COPD rats, while cell apoptosis, caspase-3, and Cyto C were increased (P<0.01).
All aforementioned parameters were improved in treatment groups (P<0.05).
ATP, MMP, and mPTPs were significantly higher in the Bufei Jianpi group than in the aminophylline group, while cell apoptosis, caspase-3, and Cyto C were lower (P<0.05).
Conclusions.
Bufei Jianpi granules can inhibit mitochondrial dysfunction and cell apoptosis in peripheral muscles, which might be the mechanism involved in improving skeletal muscle function in COPD patients.
American Psychological Association (APA)
Mao, Jing& Ya, Li& Li, Suyun& Jian-sheng, Li& Tian, Yange& Feng, Suxiang…[et al.]. 2019. Bufei Jianpi Granules Reduce Quadriceps Muscular Cell Apoptosis by Improving Mitochondrial Function in Rats with Chronic Obstructive Pulmonary Disease. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1148420
Modern Language Association (MLA)
Mao, Jing…[et al.]. Bufei Jianpi Granules Reduce Quadriceps Muscular Cell Apoptosis by Improving Mitochondrial Function in Rats with Chronic Obstructive Pulmonary Disease. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1148420
American Medical Association (AMA)
Mao, Jing& Ya, Li& Li, Suyun& Jian-sheng, Li& Tian, Yange& Feng, Suxiang…[et al.]. Bufei Jianpi Granules Reduce Quadriceps Muscular Cell Apoptosis by Improving Mitochondrial Function in Rats with Chronic Obstructive Pulmonary Disease. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1148420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148420