Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic β Cell Identity in dbdb Mice
Joint Authors
Lu, Fuer
Gong, Jing
Yuan, Fen
Wang, Dingkun
Ma, Leyi
Qin, Xin
Hu, Meilin
Su, Hao
Dong, Hui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Huanglian-Renshen-Decoction (HRD) is widely used to treat type 2 diabetes mellitus (T2DM) in China.
However, the underlying mechanism is unclear.
We aimed to investigate the mechanism by which HRD regulates the glucose level.
Forty 7-8-week-old db/db (BSK) mice were randomly assigned to the following four groups: model, low dose HRD (LHRD), high dose HRD (HHRD), and saxagliptin (SAX).
Additionally, 10 db/m mice were assigned to control group.
The experimental mice were administered 3.03g/kg/d and 6.06g/kg/d of HRD in the LHRD and HHRD groups, respectively, and 10mg/kg/d saxagliptin in the SAX group for 8 weeks.
The control and model groups were supplied with distilled water.
After the intervention, the pancreas and blood were collected and tested.
Compared with that of model group, the fasting blood glucose (FBG) was significantly decreased in all intervention groups (p < 0.05 or 0.01), whereas fasting serum insulin (FINS) was increased significantly in both HHRD and SAX groups.
The immunofluorescence images showed that the mass of insulin+ cells was increased and that of glucagon+ cells was reduced obviously in experimental groups compared to those of the model group.
In addition, the coexpression of insulin, glucagon, and PDX1 was decreased in HHRD group, and the level of caspase 12 in islet was decreased significantly in all intervention groups.
However, little difference was found in the number and morphology of islet, and the expression of ki67, bcl2, bax, caspase 3, and cleaved-caspase 3 in the pancreas among groups.
Interestingly, the cleaved-Notch1 level was increased and the Ngn3 level in islet was decreased significantly in HHRD group.
The HRD showed dose-dependent effects on glucose metabolism improvement through maintenance of β cell identity via a mechanism that might involve the Notch1/Ngn3 signal pathway in db/db mice.
American Psychological Association (APA)
Yuan, Fen& Wang, Dingkun& Ma, Leyi& Qin, Xin& Gong, Jing& Hu, Meilin…[et al.]. 2019. Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic β Cell Identity in dbdb Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148425
Modern Language Association (MLA)
Yuan, Fen…[et al.]. Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic β Cell Identity in dbdb Mice. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1148425
American Medical Association (AMA)
Yuan, Fen& Wang, Dingkun& Ma, Leyi& Qin, Xin& Gong, Jing& Hu, Meilin…[et al.]. Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic β Cell Identity in dbdb Mice. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148425
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148425