Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model
Joint Authors
Pan, Lin-Lin
Sun, Qi-Hui
Liu, Gui-Rong
Guo, Jia-Yin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-30
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The hypoglycemic decoction (HD) is a traditional Chinese medicine (TCM) preparation for the treatment of diabetes mellitus (DM), with a remarkable therapeutic effect.
However, its mechanism of action is still unclear at the metabolic level.
In this study, the biochemical markers from type 2 DM (T2DM) rats, induced by a high-sugar and high-fat diet combined with streptozotocin (STZ), were detected.
The metabolomics-based analysis using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was conducted to evaluate urine samples from control, model, metformin, and HD groups.
After oral administration of HD for 28 days, the general state, weight, fasting blood glucose (FBG), blood lipid level, oral glucose tolerance test (OGTT), fasting insulin (FINS), insulin sensitivity index (ISI), and homeostasis model assessment of insulin resistance (HOMA-IR) were significantly improved (P<0.01).
The western blotting showed that HD can enhance the protein expression of glucose transporter 4 (GLUT4) and adenosine monophosphate-activated protein kinase (AMPK).
The metabolomics results revealed that after treatment with HD, the levels of L-carnitine, 1-methyladenosine, 1-methylhistamine, and 3-indoleacrylic acid were upregulated and the levels of riboflavin, phenylalanine, atrolactic acid, 2-oxoglutarate, citrate, isocitrate, cortisol, and glucose were downregulated.
The main mechanism may be closely related to the regulation of the tricarboxylic acid (TCA) cycle, phenylalanine metabolism, glyoxylate metabolism, and dicarboxylate metabolism.
Additionally, it was also found that HD can regulate the protein expression of GLUT4 and AMPK to interfere with TCA cycle and carbohydrate metabolism to treat T2DM.
American Psychological Association (APA)
Pan, Lin-Lin& Sun, Qi-Hui& Liu, Gui-Rong& Guo, Jia-Yin. 2019. Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1148535
Modern Language Association (MLA)
Pan, Lin-Lin…[et al.]. Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1148535
American Medical Association (AMA)
Pan, Lin-Lin& Sun, Qi-Hui& Liu, Gui-Rong& Guo, Jia-Yin. Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1148535
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148535