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Jieduquyuziyin Prescription-Treated Rat Serum Suppresses Activation of Peritoneal Macrophages in MRLLpr Lupus Mice by Inhibiting IRAK1 Signaling Pathway
Joint Authors
Ji, Lina
Hou, Xiaoli
Deng, Xian
Fan, Xuemin
Zhuang, Aiwen
Zhang, Xiufeng
Li, Rongqun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, and Jieduquyuziyin prescription (JP) is a traditional Chinese medicine (TCM) formula that has been testified to be effective for SLE treatment as an approved hospital prescription for many years in China.
However, its mechanism of action in the treatment of this disease is largely unknown.
The purpose of this study was to determine whether JP-treated rat serum can inhibit the activation of peritoneal macrophages in MRL/lpr mice by downregulating the IRAK1 signaling pathway, thereby achieving the effect of improving SLE.
The JP-treated rat serum was prepared, and the peritoneal macrophages of MRL/lpr lupus mice were isolated in vitro, and the effect of JP on cell viability was detected by the CCK8 method.
After LPS induction and shRNA lentiviral transfection, the effect of JP on the expression of IRAK1 in cells was detected by immunofluorescence staining.
The content of TNF-α and IL-6 in the cell supernatant was determined by ELISA.
The expression of IRAK1, NF-κB, TNF-α, and IL-6 mRNA was detected by RT-PCR, and the expression levels of IRAK1, p-IRAK1, TRAF6, IKBα, p-IKBα, IKK + IKK, NF-κB, and p-NF-κB proteins was detected by western blot method.
We investigated the role of JP in peritoneal macrophages of the MRL/lpr mouse and identified the possible mechanisms of action.
The results showed that JP could reduce the phosphorylation of IRAK1 and its downstream proteins induced by LPS and inhibit the expression of inflammatory cytokines, including TNF-α and IL-6.
In addition, after the transfection of cells with shRNA lentiviral, the results of JP tended to be consistent.
In conclusion, JP may inhibit the activation of peritoneal macrophages in MRL/lpr mice by downregulating the IRAK1-NF-κB signaling pathway, and IRAK1 may be a potential target for JP treatment of SLE.
American Psychological Association (APA)
Ji, Lina& Hou, Xiaoli& Deng, Xian& Fan, Xuemin& Zhuang, Aiwen& Zhang, Xiufeng…[et al.]. 2019. Jieduquyuziyin Prescription-Treated Rat Serum Suppresses Activation of Peritoneal Macrophages in MRLLpr Lupus Mice by Inhibiting IRAK1 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148901
Modern Language Association (MLA)
Ji, Lina…[et al.]. Jieduquyuziyin Prescription-Treated Rat Serum Suppresses Activation of Peritoneal Macrophages in MRLLpr Lupus Mice by Inhibiting IRAK1 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1148901
American Medical Association (AMA)
Ji, Lina& Hou, Xiaoli& Deng, Xian& Fan, Xuemin& Zhuang, Aiwen& Zhang, Xiufeng…[et al.]. Jieduquyuziyin Prescription-Treated Rat Serum Suppresses Activation of Peritoneal Macrophages in MRLLpr Lupus Mice by Inhibiting IRAK1 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148901
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148901