The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography
Joint Authors
Chang, Yanxu
Li, Jin
He, Jun
Hao, Jia
Chen, Xiaopeng
Ge, Yuanyuan
Chen, Shujing
Luo, Qian
Wang, Chun-peng
Yang, Xuejing
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Angelicae pubescentis radix (APR) is widely applied in treating rheumatoid arthritis in China.
Coumarins are the major active compounds of APR extract including columbianetin, columbianetin acetate, osthole, and columbianadin.
The in vivo behavior of the four major coumarins of APR has not been systematically reported.
A feasible and reliable ultra-performance liquid chromatography (UPLC) method was established and validated for the quantification of the above four coumarins in rat various tissues (including heart, liver, spleen, lung, kidney, uterus, ovary, and muscle) after oral administration of APR extract.
The separation was implemented on a Waters ACQUITY BEH C18 column (4.6 mm × 100 mm, 1.7 μm) with gradient mobile phase comprising acetonitrile-water (with 1mM formic acid) at a flow rate of 0.3 mL/min.
The tissue homogenate samples were prepared by liquid-liquid extraction with ethyl acetate.
The calibration curves were linear in the range of 1.6-20000 ng/mL for four coumarins with the lower limit of quantitation of 1.6 ng/mL in rat tissues.
The intraday and interday precisions and recoveries were all within 80-100% with the relative standard deviations (RSDs) which were all less than 10.9%.
The method was successfully applied to the tissue distribution research after oral administration of 6.0 g/kg APR extract to rat.
The results revealed that the tissues distributions of four coumarins were in the liver, followed by the ovary, uterus, kidney, lung, heart, spleen, and muscle.
American Psychological Association (APA)
Ge, Yuanyuan& Chen, Shujing& Luo, Qian& Wang, Chun-peng& Hao, Jia& He, Jun…[et al.]. 2019. The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1148912
Modern Language Association (MLA)
Ge, Yuanyuan…[et al.]. The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1148912
American Medical Association (AMA)
Ge, Yuanyuan& Chen, Shujing& Luo, Qian& Wang, Chun-peng& Hao, Jia& He, Jun…[et al.]. The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1148912
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148912