Berberine Modulates LPA Function to Inhibit the Proliferation and Inflammation of FLS-RA via p38ERK MAPK Pathway Mediated by LPA1
Joint Authors
Chen, Zhe
Huang, Ying
Wang, Hui
Yang, Sisi
Shen, Pan
Huang, Yao
Ba, Xin
Lin, Weiji
Wang, Yu
Qin, Kai
Tu, Shenghao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
This study aimed to investigate whether berberine exerted anti-inflammatory and antiproliferative effects on the fibroblast-like synoviocytes of rheumatoid arthritis (FLS-RA) through regulating the lysophosphatidic acid (LPA) function.
Methods.
Firstly, the expression levels of LPA and lysophosphatidic acid receptor 1 (LPA1) in RA patients, osteoarthritis (OA) patients, and healthy controls were detected.
Moreover, molecular docking was employed to characterize the binding sites of berberine in the predicted protein targets.
Later, FLS-RA were stimulated using berberine, LPA, and the specific inhibitor (Ki16425) of LPA1, thereafter, the effects on the proliferation, apoptosis, the release of inflammatory mediators of FLS-RA, and the MAPK pathway were observed.
Results.
Compared with healthy controls (n = 25), the plasma LPA level (n = 28) and synovial fluid (n = 10) were markedly higher in RA patients.
LPA1 was highly expressed in RA patients (n = 4) relative to that in OA patients (n = 4).
Berberine remarkably inhibited the proliferation and the excessive production of IL-6 and TNF-α in FLS-RA, whereas suppressing the expression of K-ras, c-Raf, and p-38/ERK-phosphorylation.
In addition, berberine inhibited the LPA-induced p-38/ERK-phosphorylation through binding to LPA1.
Conclusions.
LPA plays a certain role in promoting the proliferation and inflammation of FLS-RA.
Berberine potentially modulates LPA function to suppress the proliferation and inflammation of FLS-RA through blocking the p38/ERK MAPK pathway mediated by LPA1.
These findings suggest that, berberine possesses potential lipid-regulating, antiarthritis, and synovial hyperplasia inhibition activities against RA, which may provide a promising therapeutic target for the clinical drug development for RA patients with dyslipidemia and high CVD risk.
American Psychological Association (APA)
Wang, Hui& Tu, Shenghao& Yang, Sisi& Shen, Pan& Huang, Yao& Ba, Xin…[et al.]. 2019. Berberine Modulates LPA Function to Inhibit the Proliferation and Inflammation of FLS-RA via p38ERK MAPK Pathway Mediated by LPA1. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1148985
Modern Language Association (MLA)
Wang, Hui…[et al.]. Berberine Modulates LPA Function to Inhibit the Proliferation and Inflammation of FLS-RA via p38ERK MAPK Pathway Mediated by LPA1. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1148985
American Medical Association (AMA)
Wang, Hui& Tu, Shenghao& Yang, Sisi& Shen, Pan& Huang, Yao& Ba, Xin…[et al.]. Berberine Modulates LPA Function to Inhibit the Proliferation and Inflammation of FLS-RA via p38ERK MAPK Pathway Mediated by LPA1. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1148985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148985