Inhibition of Cytochrome P450 Activities by Sophora flavescens Extract and Its Prenylated Flavonoids in Human Liver Microsomes
Joint Authors
Shin, Jae-Gook
Yim, Daeun
Kim, Min Jung
Shin, Yumi
Lee, Su-Jun
Kim, Dong Hyun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sophora flavescens possesses several pharmacological properties and has been widely used for the treatment of diarrhea, inflammation, abscess, dysentery, and fever in East Asian countries.
S.
flavescens is a major source of prenylated flavonoids, such as sophoraflavone and kushenol.
In this study, we examined the effects of S.
flavescens extract and its prenylated flavonoids on cytochrome P450 (CYP) isoform activity in human liver microsomes.
The extract inhibited CYP2C8, CYP2C9, CYP2C19, and CYP3A activities, with IC50 values of 1.42, 13.6, 19.1, and 50 µg/mL, respectively.
CYP2B6 was only inhibited in human liver microsomes preincubated with the extract.
CYP3A4 was more strongly inhibited by the extract in the presence of NADPH, suggesting that the extract may inhibit CYP2B6 and CYP3A4 via mechanism-based inactivation.
Prenylated flavonoids also inhibited CYP isoforms with different selectivity and modes of action.
Kushenol I, leachianone A, and sophoraflavone G inhibited CYP2B6, whereas kushenol C, kushenol I, kushenol M, leachianone A, and sophoraflavone G inhibited CYP3A4 via mechanism-based inhibition.
Our results suggest that S.
flavescens may contribute to herb–drug interactions when coadministered with drugs metabolized by CYP2B6, CYP2C8, CYP2C9, and CYP3A4.
American Psychological Association (APA)
Yim, Daeun& Kim, Min Jung& Shin, Yumi& Lee, Su-Jun& Shin, Jae-Gook& Kim, Dong Hyun. 2019. Inhibition of Cytochrome P450 Activities by Sophora flavescens Extract and Its Prenylated Flavonoids in Human Liver Microsomes. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1149037
Modern Language Association (MLA)
Yim, Daeun…[et al.]. Inhibition of Cytochrome P450 Activities by Sophora flavescens Extract and Its Prenylated Flavonoids in Human Liver Microsomes. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1149037
American Medical Association (AMA)
Yim, Daeun& Kim, Min Jung& Shin, Yumi& Lee, Su-Jun& Shin, Jae-Gook& Kim, Dong Hyun. Inhibition of Cytochrome P450 Activities by Sophora flavescens Extract and Its Prenylated Flavonoids in Human Liver Microsomes. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1149037
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149037