Systematically Characterizing Chemical Profile and Potential Mechanisms of Qingre Lidan Decoction Acting on Cholelithiasis by Integrating UHPLC-QTOF-MS and Network Target Analysis
Joint Authors
Leng, Aijing
Huang, Peng
Ke, Hongwen
Qiu, Yang
Cai, Mingchen
Qu, Jialin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-03
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Qingre Lidan Decoction (QRLDD), a classic precompounded prescription, is widely used as an effective treatment for cholelithiasis clinically.
However, its chemical profile and mechanism have not been characterized and elucidated.
In the present study, a rapid, sensitive, and reliable ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established for comprehensively identifying the major constituents in QRLDD.
Furthermore, a network pharmacology strategy based on the chemical profile was applied to clarify the synergetic mechanism.
A total of 72 compounds containing flavonoids, terpenes, phenolic acid, anthraquinones, phenethylalchohol glycosides, and other miscellaneous compounds were identified, respectively.
410 disease genes, 432 compound targets, and 71 related pathways based on cholelithiasis-related and compound-related targets databases as well as related pathways predicted by the Kyoto Encyclopedia of Genes and Genomes database were achieved.
Among these pathways and genes, pathway in cancer and MAPK signaling pathway may play an important role in the development of cholelithiasis.
EGFR may be a crucial target in the conversion of gallstones to gallbladder carcinoma.
Regulation of PRKCB/RAF1/MAP2K1/MAPK1 is associated with cell proliferation and differentiation.
Thus, the fingerprint coupled with network pharmacology analysis could contribute to simplifying the complex system and providing directions for further research of QRLDD.
American Psychological Association (APA)
Huang, Peng& Ke, Hongwen& Qiu, Yang& Cai, Mingchen& Qu, Jialin& Leng, Aijing. 2019. Systematically Characterizing Chemical Profile and Potential Mechanisms of Qingre Lidan Decoction Acting on Cholelithiasis by Integrating UHPLC-QTOF-MS and Network Target Analysis. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1149040
Modern Language Association (MLA)
Huang, Peng…[et al.]. Systematically Characterizing Chemical Profile and Potential Mechanisms of Qingre Lidan Decoction Acting on Cholelithiasis by Integrating UHPLC-QTOF-MS and Network Target Analysis. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-19.
https://search.emarefa.net/detail/BIM-1149040
American Medical Association (AMA)
Huang, Peng& Ke, Hongwen& Qiu, Yang& Cai, Mingchen& Qu, Jialin& Leng, Aijing. Systematically Characterizing Chemical Profile and Potential Mechanisms of Qingre Lidan Decoction Acting on Cholelithiasis by Integrating UHPLC-QTOF-MS and Network Target Analysis. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1149040
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149040