Cytotoxic and Apoptotic Activity of Majoranolide from Mezilaurus crassiramea on HL-60 Leukemia Cells
Joint Authors
Cepa Matos, Maria de Fatima
Heemann, Lanna M.
de Souza, Kamylla F. S.
Tófoli, Danilo
Filippin, Kelly J.
Garcez, Walmir S.
Garcez, Fernanda R.
Perdomo, Renata T.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Majoranolide, a butanolide isolated from the nonpolar fraction of an ethanol extract of Mezilaurus crassiramea (Lauraceae) fruits, is being reported for the first time in this genus and the third time in plants.
Structurally identified from 1D and 2D NMR and HRESIMS data, majoranolide proved cytotoxic against cancer cells—MCF-7 and MDA-MB-231 (breast), HT-29 (colon), PC-3 (prostate), 786-0 (renal), and HL-60 (leukemia)—inhibiting growth in HL-60 cells (GI50 = 0.21 μM) and exhibiting higher selectivity for this line than for nonneoplastic NIH/3T3 murine fibroblasts.
Effects on the cell cycle, caspase-3 activation, and plasma membrane integrity were evaluated by flow cytometry.
Expression of genes related to apoptotic pathways (BAX, BCL2, BIRC5, and CASP8) was investigated using RT-qPCR.
At 50 μM, majoranolide induced cell cycle arrest at G1 in 24 h increased the sub-G1 population in 48 h and increased caspase-3 activation in a time-dependent manner.
The compound upregulated BAX and CASP8 transcription (proapoptotic genes) and downregulated BIRC5 (antiapoptotic).
Loss of plasma membrane integrity in 30% of cells occurred at 48 h, but not at 24 h, characterizing gradual, programmed death.
The results suggest that majoranolide cytotoxicity involves apoptosis induction in HL-60 cells, although other mechanisms may contribute to this cell death.
American Psychological Association (APA)
Heemann, Lanna M.& de Souza, Kamylla F. S.& Tófoli, Danilo& Filippin, Kelly J.& Garcez, Walmir S.& Cepa Matos, Maria de Fatima…[et al.]. 2019. Cytotoxic and Apoptotic Activity of Majoranolide from Mezilaurus crassiramea on HL-60 Leukemia Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1149420
Modern Language Association (MLA)
Heemann, Lanna M.…[et al.]. Cytotoxic and Apoptotic Activity of Majoranolide from Mezilaurus crassiramea on HL-60 Leukemia Cells. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1149420
American Medical Association (AMA)
Heemann, Lanna M.& de Souza, Kamylla F. S.& Tófoli, Danilo& Filippin, Kelly J.& Garcez, Walmir S.& Cepa Matos, Maria de Fatima…[et al.]. Cytotoxic and Apoptotic Activity of Majoranolide from Mezilaurus crassiramea on HL-60 Leukemia Cells. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1149420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149420