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iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
Joint Authors
Yang, Bing-You
Guan, Wei
Liu, Yan
Li, Xiaomao
Kuang, Haixue
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Kidney-yin deficiency syndrome (KYDS) is a typical syndrome encountered in traditional Chinese medicine (TCM) and is characterized by impaired lipid and glucose homeostasis.
The hypothalamus acts as an important regulatory organ by controlling lipid and glucose metabolism in the body.
Therefore, proteins in the hypothalamus could play important roles in KYDS development; however, the mechanisms responsible for KYDS remain unclear.
Herein, iTRAQ-based proteomics was performed to analyze the protein expression in the hypothalamus of KYDS rats induced by levothyroxine (L-T4).
Results revealed a total of 44 downregulated and 18 upregulated proteins in KYDS group relative to the control group.
Gene Ontology (GO) analysis revealed that the differently expressed proteins (DEPs) were related to single-organism metabolism process under the biological process (BP), extracellular region part and organelle under the cellular component (CC), and oxidoreductase activity under the molecular function (MF).
Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis showed that fatty acid degradation and pyruvate metabolism participated in the metabolism regulation in KYDS rats.
RT-PCR validation of five distinctly expressed proteins related to the two pathways was consistent with the results of proteomics analysis.
Taken together, the inhibition of fatty acid degradation and pyruvate metabolism in hypothalamus could potentially cause the dysfunction of the lipid and glucose metabolism in KYDS rats.
This current study identified some novel potential biomarkers of KYDS and provided the basis for further research of KYDS.
American Psychological Association (APA)
Guan, Wei& Liu, Yan& Li, Xiaomao& Yang, Bing-You& Kuang, Haixue. 2019. iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149539
Modern Language Association (MLA)
Guan, Wei…[et al.]. iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1149539
American Medical Association (AMA)
Guan, Wei& Liu, Yan& Li, Xiaomao& Yang, Bing-You& Kuang, Haixue. iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149539
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149539