Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOFMS Analysis
Joint Authors
Wang, Dongsheng
Qiu, Xinjian
Wang, Wenbo
Nie, Shanshan
Zhao, Yuhang
Yao, Ye
Yi, Min
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-23
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Recently, metabolomic methods have been used to explore the complex pathogenesis of cancer and the mechanism of action of traditional Chinese medicine (TCM) formulae.
In this study, first, modified Si Jun Zi Tang (MSJZT) was prepared with strict quality control using the instrument method of ultra performance liquid chromatography and photodiode array detector (UPLC-PDA).
Subsequently, in vivo experiments with tumour-bearing nude mice demonstrated that MSJZT exerted good antitumour effects.
MSJZT not only significantly increased mouse body weight but also shrank the tumour volume.
Then, the HILIC UHPLC-Q-TOF/MS-based metabolomics approach was used for exploring the pathogenesis of gastric cancer and the molecular mechanism of MSJZT.
A total of 59 potential biomarkers in plasma were identified, and 6 pathways were found to be disturbed in gastric cancer.
In contrast, after 3 weeks of MSJZT intervention, 32 potential biomarkers were identified, and 4 altered pathways were detected.
The changes in glycolytic, amino acid, and lipid metabolisms could be partially regulated by MSJZT through decreasing the content of lactic dehydrogenase (LDH), glutamine synthetase (GS), phosphocholine cytidylyltransferase (PCYT2) mRNA, and protein level.
In conclusion, we established a HILIC UHPLC-Q-TOF/MS metabolomic analysis method to demonstrate a complex metabolic profile of gastric cancer.
The disordered metabolism could be partially regulated by MSJZT.
These findings not only establish a solid foundation for TCM to treat gastric cancer but also provide a basis for further exploration of the precise mechanism of MSJZT activity.
American Psychological Association (APA)
Nie, Shanshan& Zhao, Yuhang& Qiu, Xinjian& Wang, Wenbo& Yao, Ye& Yi, Min…[et al.]. 2019. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOFMS Analysis. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1149607
Modern Language Association (MLA)
Nie, Shanshan…[et al.]. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOFMS Analysis. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1149607
American Medical Association (AMA)
Nie, Shanshan& Zhao, Yuhang& Qiu, Xinjian& Wang, Wenbo& Yao, Ye& Yi, Min…[et al.]. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOFMS Analysis. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1149607
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149607