Design, Preparation, and Characterization of Dioscin Nanosuspensions and Evaluation of Their Protective Effect against Carbon Tetrachloride-Induced Acute Liver Injury in Mice
Joint Authors
Song, Xiao
JU, Hong-Ye
Hu, Kun-Xia
Zhao, Guo-Wang
Tang, Zhi-Shu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-14
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The purpose of this study was to prepare a dioscin nanosuspension (Dio-NS) that has a better distance and high solubility for oral administration and to evaluate its hepatoprotective effects.
Optimal primary manufacture parameters, including shear time, shear speed, emulation temperature, pressure, and cycles of homogenization, were determined by single-factor experiments.
The concentrations of dioscin, SDS, and soybean lecithin were optimized using the central composite design-response surface method, and their effects on the mean particle size (MPS) and particle size distribution of Dio-NS were investigated.
Characterization of the Dio-NS formulations included examinations of the surface morphology and physical status of dioscin in Dio-NS, the stability of Dio-NS at different temperatures, in vitro solubility, and liver protective effect in vivo.
Under optimal conditions, Dio-NS had an MPS of 106.72 nm, polydispersity index of 0.221, and zeta potential of −34.27 mV.
Furthermore, the proportion of dioscin in Dio-NS was approximately 21.26%.
The observation of particles with a spherical shape and the disappearance of crystalline peaks indicated that the physical and chemical properties of Dio-NS were altered.
Furthermore, we observed that the dissolution of Dio-NS was superior to that of a physical mixture and Dio-GZF.
Moreover, Dio-NS was demonstrated to have a protective effect against CCl4-induced acute liver damage in mice that was equivalent to that of silymarin (a positive control drug) at the same dose.
The good hepatoprotective effect of our Dio-NS preparation can provide a theoretical basis for investigating its absorption mechanisms in the body.
American Psychological Association (APA)
JU, Hong-Ye& Hu, Kun-Xia& Zhao, Guo-Wang& Tang, Zhi-Shu& Song, Xiao. 2019. Design, Preparation, and Characterization of Dioscin Nanosuspensions and Evaluation of Their Protective Effect against Carbon Tetrachloride-Induced Acute Liver Injury in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1149650
Modern Language Association (MLA)
JU, Hong-Ye…[et al.]. Design, Preparation, and Characterization of Dioscin Nanosuspensions and Evaluation of Their Protective Effect against Carbon Tetrachloride-Induced Acute Liver Injury in Mice. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1149650
American Medical Association (AMA)
JU, Hong-Ye& Hu, Kun-Xia& Zhao, Guo-Wang& Tang, Zhi-Shu& Song, Xiao. Design, Preparation, and Characterization of Dioscin Nanosuspensions and Evaluation of Their Protective Effect against Carbon Tetrachloride-Induced Acute Liver Injury in Mice. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1149650
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149650