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Rho-Kinase II Inhibitory Potential of Eurycoma longifolia New Isolate for the Management of Erectile Dysfunction
Joint Authors
Ezzat, Shahira M.
Mohamed, Shanaz O.
Okba, Mona M.
Ezzat, Marwa I.
Aborehab, Nora M.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Eurycoma longifolia Jack (Fam.: Simaroubaceae), known as Tongkat Ali (TA), has been known as a symbol of virility and sexual power.
The aim of the study was to screen E.
longifolia aqueous extract (AE) and isolates for ROCK-II inhibition.
Results.
The AE (1-10 μg/ml) showed a significant inhibition for ROCK-II activity (62.8-81%) at P < 0.001 with an IC50 (651.1 ± 32.9 ng/ml) compared to Y-27632 ([(+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride]) (68.15-89.9 %) at same concentrations with an IC50 (192 ± 8.37 ng/ml).
Chromatographic purification of the aqueous extract (AE) allowed the isolation of eight compounds; stigmasterol T1, trans-coniferyl aldehyde T2, scopoletin T3, eurycomalactone T4, 6α- hydroxyeurycomalactone T5, eurycomanone T6, eurycomanol T7, and eurycomanol-2-O-β-D-glucopyranoside T8.
This is the first report for the isolation of T1 and T3 from E.
longifolia and for the isolation of T2 from genus Eurycoma.
The isolates (at 10 μg/ml) exhibited maximum inhibition % of ROCK-II 82.1 ± 0.63 (T2), 78.3 ± 0.38 (T6), 77.1 ± 0.11 (T3), 76.2 ± 3.53 (T4), 74.5 ± 1.27 (T5), 74.1 ± 2.97 (T7), 71.4 ± 2.54 (T8), and 60.3 ± 0.14 (T1), where the newly isolated compound trans-coniferyl aldehyde T2 showed the highest inhibitory activity among the tested isolated compounds and even higher than the total extract AE.
The standard Y-27632 (10 μg/ml) showed 89.9 ± 0.42 % inhibition for ROCK-II activity when compared to control at P < 0.0001.
Conclusion.
The traditional use of E.
longifolia as aphrodisiac and for male sexual disorders might be in part due to the ROCK-II inhibitory potential.
American Psychological Association (APA)
Ezzat, Shahira M.& Okba, Mona M.& Ezzat, Marwa I.& Aborehab, Nora M.& Mohamed, Shanaz O.. 2019. Rho-Kinase II Inhibitory Potential of Eurycoma longifolia New Isolate for the Management of Erectile Dysfunction. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1149771
Modern Language Association (MLA)
Ezzat, Shahira M.…[et al.]. Rho-Kinase II Inhibitory Potential of Eurycoma longifolia New Isolate for the Management of Erectile Dysfunction. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1149771
American Medical Association (AMA)
Ezzat, Shahira M.& Okba, Mona M.& Ezzat, Marwa I.& Aborehab, Nora M.& Mohamed, Shanaz O.. Rho-Kinase II Inhibitory Potential of Eurycoma longifolia New Isolate for the Management of Erectile Dysfunction. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1149771
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149771