Proteome Profiling of Membrane-Free Stem Cell Components by Nano-LSMS Analysis and Its Anti-Inflammatory Activity
Joint Authors
Heo, Jeong-Doo
Venkatarame Gowda Saralamma, Venu
Vetrivel, Preethi
Kim, Seong Min
Ha, Sang Eun
Lee, Ho Jeong
Lee, Sang Joon
Kim, Young Sil
Pak, Jung Eun
Lee, Hye Jin
Kim, Gon-Sup
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The use of adipose-derived stem cells (ADSCs) to enhance wound healing and tissue regeneration is progressively being accepted.
Proteomic profiling of cultured ADSCs by mass spectrometry (MS) is a valuable tool to determine the identity of the proteins involved in multiple pathways, which make these ADSCs unique.
In the current study, Nano-LC-MS/MS analysis was implemented on the membrane-free stem cell component (MFSCC), and the MS analysis revealed the presence of 252 proteins, that are involved in several biological functions, like metabolic process, biological regulation, developmental process, cell proliferation, and many more.
Furthermore, bioinformatic analyses of the identified proteins in MFSCC found them to be involved in versatile pathways, like integrin pathway and wound healing response-related pathways.
In addition, we also investigated the anti-inflammatory effects of MFSCC on lipopolysaccharide (LPS) stimulated mouse macrophage (RAW264.7) cells.
The cell cytotoxicity of MFSCC was measured using MTT and LDH assays, the production of nitric oxide (NO) was measured by the Griess assay, and the protein expression levels of inducible nitric oxide (iNOS) and cyclooxygenase (COX-2) were examined by western blot analysis.
The results showed that MFSCC concentrations ranging from 0.1 to 3 μg/mL did not show any significant cytotoxicity in LPS-induced RAW264.7 cells.
Treatment with MFSCC of LPS-stimulated RAW264.7 cells significantly suppressed the production of NO and the expression of iNOS and COX-2 proteins related to inflammation.
The present findings lead to a better understanding of the therapeutic potential of MFSCC and strongly promote it for the future clinical development of novel non-cell-based stem cell therapeutics.
American Psychological Association (APA)
Venkatarame Gowda Saralamma, Venu& Vetrivel, Preethi& Kim, Seong Min& Ha, Sang Eun& Lee, Ho Jeong& Lee, Sang Joon…[et al.]. 2019. Proteome Profiling of Membrane-Free Stem Cell Components by Nano-LSMS Analysis and Its Anti-Inflammatory Activity. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149878
Modern Language Association (MLA)
Venkatarame Gowda Saralamma, Venu…[et al.]. Proteome Profiling of Membrane-Free Stem Cell Components by Nano-LSMS Analysis and Its Anti-Inflammatory Activity. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1149878
American Medical Association (AMA)
Venkatarame Gowda Saralamma, Venu& Vetrivel, Preethi& Kim, Seong Min& Ha, Sang Eun& Lee, Ho Jeong& Lee, Sang Joon…[et al.]. Proteome Profiling of Membrane-Free Stem Cell Components by Nano-LSMS Analysis and Its Anti-Inflammatory Activity. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149878
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149878