Anti-Inflammatory Effects of Licania macrocarpa Cuatrec Methanol Extract Target Src- and TAK1-Mediated Pathways
Joint Authors
Kim, Eunji
Aziz, Nur
Shin, Kon Kuk
Hong, Yo Han
Park, Sang Hee
Kim, Sunggyu
Cho, Jae Youl
Park, Jae Gwang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In this study, we investigated the anti-inflammatory effects of Licania macrocarpa Cuatrec methanol extract (Lm-ME) in vitro and in vivo and found pharmacological target proteins of Lm-ME in TLR4-mediated inflammatory signaling.
This extract reduced NO production and mRNA expression of inflammatory cytokines such as iNOS, COX-2, IL-6, and IL-1β.
In the NF-κB- and AP-1-mediated luciferase reporter gene assay, transcription factor activities decreased under cotransfection with MyD88 or TRIF.
Phosphorylated protein levels of Src, PI3K, IKKα/β, and IκBα as well as p50 and p65 in the NF-κB signal pathway were downregulated, and phosphorylation of TAK1, MEK1/2, MKK4/7, and MKK3/6 as well as ERK, JNK, and p38 was decreased in the AP-1 signal pathway.
Through overexpression of HA-Src and HA-TAK1, respectively, Lm-ME inhibited autophosphorylation of overexpressed proteins and thereby activated fewer downstream signaling molecules.
Lm-ME also attenuated stomach ulcers in an HCl/EtOH-induced acute gastritis model mice, and COX-2 mRNA expression and phosphorylated TAK1 levels in gastric tissues were diminished.
The flavonoids kaempferol and quercetin were identified in the HPLC analysis of Lm-ME; both are actively anti-inflammatory.
Therefore, these results suggest that Lm-ME can be used for anti-inflammatory remedy by targeting Src and TAK1.
American Psychological Association (APA)
Shin, Kon Kuk& Park, Jae Gwang& Hong, Yo Han& Aziz, Nur& Park, Sang Hee& Kim, Sunggyu…[et al.]. 2019. Anti-Inflammatory Effects of Licania macrocarpa Cuatrec Methanol Extract Target Src- and TAK1-Mediated Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1149937
Modern Language Association (MLA)
Shin, Kon Kuk…[et al.]. Anti-Inflammatory Effects of Licania macrocarpa Cuatrec Methanol Extract Target Src- and TAK1-Mediated Pathways. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1149937
American Medical Association (AMA)
Shin, Kon Kuk& Park, Jae Gwang& Hong, Yo Han& Aziz, Nur& Park, Sang Hee& Kim, Sunggyu…[et al.]. Anti-Inflammatory Effects of Licania macrocarpa Cuatrec Methanol Extract Target Src- and TAK1-Mediated Pathways. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1149937
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149937