WS-5 Extract of Curcuma longa, Chaenomeles sinensis, and Zingiber officinale Contains Anti-AChE Compounds and Improves β-Amyloid-Induced Memory Impairment in Mice
Joint Authors
Kim, Dae Sung
Lee, Jeong-Ho
Cho, Hyoung-Kwon
Kim, Ju Eun
Shrestha, Abinash Chandra
Kim, Hyo Shin
Ham, Ha Neul
Kim, Jun Hyeong
Kim, Yeong Jee
Noh, Yun Jeong
Kim, Su Jin
Kim, Dae Keun
Moon, Kwang Hyun
Jeong, Kyung Ok
Leem, Jae Yoon
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-01
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (Aβ) as senile plaques associated with reactive astrocytes and microglial activation in the brain.
The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against Aβ-induced AD in mice and to identify the extract’s active constituents.
In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE).
Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented Aβ oligomerization via inhibition of Aβ1-42 aggregation.
Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS).
Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells.
WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-α and IL-6.
The memory impairment in mice with Aβ-induced AD was studied using the Morris water maze and passive avoidance test.
Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain.
The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced Aβ plaque accumulation in Aβ-induced AD.
HPLC analysis identified the extract’s active compounds that exert anti-AChE activity.
In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.
American Psychological Association (APA)
Kim, Ju Eun& Shrestha, Abinash Chandra& Kim, Hyo Shin& Ham, Ha Neul& Kim, Jun Hyeong& Kim, Yeong Jee…[et al.]. 2019. WS-5 Extract of Curcuma longa, Chaenomeles sinensis, and Zingiber officinale Contains Anti-AChE Compounds and Improves β-Amyloid-Induced Memory Impairment in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1150020
Modern Language Association (MLA)
Kim, Ju Eun…[et al.]. WS-5 Extract of Curcuma longa, Chaenomeles sinensis, and Zingiber officinale Contains Anti-AChE Compounds and Improves β-Amyloid-Induced Memory Impairment in Mice. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1150020
American Medical Association (AMA)
Kim, Ju Eun& Shrestha, Abinash Chandra& Kim, Hyo Shin& Ham, Ha Neul& Kim, Jun Hyeong& Kim, Yeong Jee…[et al.]. WS-5 Extract of Curcuma longa, Chaenomeles sinensis, and Zingiber officinale Contains Anti-AChE Compounds and Improves β-Amyloid-Induced Memory Impairment in Mice. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1150020
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150020