Effect of Moxibustion on Testosterone Secretion and Apoptosis of Spermatogenic Cells in Aging Rats
Joint Authors
Yao, Chongjie
Zhao, Chen
Zhang, Shuyan
Liu, Shimin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Analysis of androgen secretion and sperm production was conducted in the testis to investigate the efficacy of moxibustion on testicular function in aging rats.
Male Sprague–Dawley rats were randomly divided into the aging group (N = 8), the mild-warm moxibustion group (N = 8), and the youth control group (N = 8).
Rats in the mild-warm moxibustion group (MWMG) were exposed to mild-warm moxibustion at the Zusanli (ST36) and Shenshu (BL23) acupuncture points daily, from the age of 12 months until the age of 24 months.
After the intervention, testicular tissue was harvested from all rats across groups.
Changes in testicular structure were examined by hematoxylin and eosin (H&E) stain.
Detection of the apoptosis of spermatogenic cells was performed by the TUNEL assay.
Testosterone level in the testis was analyzed by the ELISA assay, and the expression of Bax, Bcl-2, and androgen receptor (AR) in the testis was evaluated by immunohistochemistry.
AR expression analysis was subsequently performed by the western blotting assay, and the detection of telomerase activity of the testis and the expression of Bax, Bcl-2, and AR mRNA were performed by real-time PCR.
Compared with the youth controls, telomerase activity in the testis, testosterone levels, expression of AR, and expression of antiapoptosis factor Bcl-2 protein and mRNA were significantly decreased (P<0.01) in the aging group.
Spermatogenic cell apoptosis (P<0.01) and proapoptotic factor Bax expression were significantly increased (P<0.01) in the aging rats compared with the youth control group.
The MWMG exhibited significant increases in testicular telomerase activity, testosterone level, AR expression, and antiapoptosis factor Bcl-2 expression (P<0.05 or P<0.01) compared with the aging group.
In this experimental group, spermatogenic apoptosis was inhibited (P<0.01) and proapoptotic factor Bax expression significantly reduced (P<0.01).
Mild-warm moxibustion can inhibit reproductive senescence by improving telomerase activity, improving AR expression, restoring testosterone, and inhibiting spermatogenic apoptosis via regulation of Bcl-2/Bax.
American Psychological Association (APA)
Yao, Chongjie& Zhao, Chen& Zhang, Shuyan& Liu, Shimin. 2019. Effect of Moxibustion on Testosterone Secretion and Apoptosis of Spermatogenic Cells in Aging Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1150033
Modern Language Association (MLA)
Yao, Chongjie…[et al.]. Effect of Moxibustion on Testosterone Secretion and Apoptosis of Spermatogenic Cells in Aging Rats. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1150033
American Medical Association (AMA)
Yao, Chongjie& Zhao, Chen& Zhang, Shuyan& Liu, Shimin. Effect of Moxibustion on Testosterone Secretion and Apoptosis of Spermatogenic Cells in Aging Rats. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1150033
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150033