![](/images/graphics-bg.png)
Effects of Xingpi Kaiyu Fang on ATP, NaK-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats
Joint Authors
Yuan, Qingjie
Li, Yang
Deng, Xiaofei
Shi, Huawei
Zhao, Zhenwu
Wang, Chunye
Feng, Xuegong
Guo, Rongjuan
Guo, Jian-You
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
To clarify the effectiveness and mechanism of the Chinese herbal formula Xingpi Kaiyu Fang (XPKYF) which is composed of American ginseng (Xi-Yang-shen), Radix curcumae (Yu-Jin), Acori tatarinowii rhizoma (Shi-Chang-pu), and Hypericum perforatum (Guan-Ye-lian-qiao) in depressed rats.
Methods.
The rat model of depression was established by chronic unpredictable mild stress (CUMS) method for 6 weeks.
Rats were randomly divided into six groups: control group, CUMS group, CUMS+XPKYF (3.6g/kg/d, 7.2g/kg/d, 14.4g/kg/d) groups, and CUMS+sertraline (4.5mg/kg/d) group.
The sucrose preference test and the forced swimming test were performed to assess the rats’ depression behavior.
Mitochondrial ultrastructure was observed by transmission electron microscope and adenosine triphosphate (ATP) content, sodium potassium ATPase (Na/K-ATPase) activity, and mitochondrial respiratory chain complexes activities in hippocampus and gastrocnemius muscle were measured at the 14th and 42nd day.
Results.
Rats subjected to six weeks of CUMS exhibited decreased sucrose preference ratio and prolonged immobility time.
CUMS reduced ATP content in hippocampus, decreased Na/K-ATPase activity and respiratory chain complex I, III, and IV activities in hippocampus and gastrocnemius muscle, and damaged mitochondrial ultrastructure of hippocampus and gastrocnemius muscle.
XPKYF at 14.4g/kg, the efficacy trend of which was better than the other drug groups, could prevent the stress-induced depressed behavior changes, inhibit the decrease of Na/K-ATPase activity in hippocampus, inhibit the decrease of respiratory chain complex III activities in hippocampus and gastrocnemius muscle, and protect mitochondria from ultrastructural damage.
Conclusions.
Energy deficiency and damaged mitochondrial ultrastructure were found in hippocampus and gastrocnemius muscle of depressed rats established by CUMS.
XPKYF could partly reverse alterations in ATP, Na/K-ATPase, and respiratory chain complexes of hippocampus and gastrocnemius muscle and protect mitochondria from ultrastructural damage.
This provides another experimental evidence for the clinical application of XPKYF in the treatment of depression.
American Psychological Association (APA)
Yuan, Qingjie& Li, Yang& Deng, Xiaofei& Shi, Huawei& Zhao, Zhenwu& Wang, Chunye…[et al.]. 2019. Effects of Xingpi Kaiyu Fang on ATP, NaK-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1150341
Modern Language Association (MLA)
Yuan, Qingjie…[et al.]. Effects of Xingpi Kaiyu Fang on ATP, NaK-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1150341
American Medical Association (AMA)
Yuan, Qingjie& Li, Yang& Deng, Xiaofei& Shi, Huawei& Zhao, Zhenwu& Wang, Chunye…[et al.]. Effects of Xingpi Kaiyu Fang on ATP, NaK-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1150341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150341