Libidibia ferrea Fruit Crude Extract and Fractions Show Anti-Inflammatory, Antioxidant, and Antinociceptive Effect In Vivo and Increase Cell Viability In Vitro
Joint Authors
Falcão, Tamires Rocha
Araújo, Aurigena Antunes de
Farias, Iuri Brilhante de
Silva, Wliana Alves Viturino da
Araújo Jr, Raimundo Fernandes de
Medeiros, Juliana Silva de
Lopes, Maria Luiza Diniz de Sousa
Guerra, Gerlane Coelho Bernardo
Ferreira, Magda Rhayanny Assunção
Soares, Luiz Alberto Lira
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-21
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Libidibia ferrea (L.
ferrea) is found throughout the northeastern region of Brazil, where it has been used in folk medicine with beneficial effects on many inflammatory disorders.
Purpose.
This study investigated the phytochemical composition of the crude extract and fractions of L.
ferrea fruit and evaluated its anti-inflammatory and antinociceptive activities in vivo and effect on cell viability in vitro.
Methods.
Characterization of polyphenols present in crude extract (CE), hydroalcoholic fractions of 20-80% ethanol (CE20, CE40, CE60, and CE80), aqueous fraction (AqF), and ethyl acetate (EAF) fractions of L.
ferrea fruit was performed by chromatographic analysis.
Anti-inflammatory activity was evaluated by using a carrageenan-induced peritonitis model submitted to a leukocyte migration assay and myeloperoxidase activity (MPO) analysis.
Total glutathione and malondialdehyde (MDA) levels were assessed to evaluate the oxidative stress level.
Antinociceptive activity was evaluated by acetic acid-induced abdominal writhing and hot plate test.
In vitro cell viability was determined by using MTT assay in a mouse embryonic fibroblast cell line (3T3 cells).
Results.
Chromatography revealed the presence of ellagic acid content in EAF (3.06), CE (2.96), and CE40 (2.89).
Gallic acid was found in EAF (12.03), CE 20 (4.43), and CE (3.99).
L.
ferrea crude extract and all fractions significantly reduced leukocyte migration and MPO activity (p<0.001).
L.
ferrea antioxidant effect was observed through high levels of total glutathione and reduction of MDA levels (p<0.001).
Acetic acid-induced nociception was significantly inhibited after administration of L.
ferrea crude extract and all fractions (p<0.001).
Crude extract and all fractions significantly increased the viability of the 3T3 cell line (p<0.05).
Conclusions.
The appropriate extraction procedure preserves the chemical components of L.
ferrea fruit, such as gallic acid and ellargic acid.
Crude extract and fractions of L.
ferrea fruit exhibited anti-inflammatory, antioxidant, antinociceptive activities in vivo and enhanced cell viability in vitro.
American Psychological Association (APA)
Falcão, Tamires Rocha& Araújo, Aurigena Antunes de& Soares, Luiz Alberto Lira& Farias, Iuri Brilhante de& Silva, Wliana Alves Viturino da& Ferreira, Magda Rhayanny Assunção…[et al.]. 2019. Libidibia ferrea Fruit Crude Extract and Fractions Show Anti-Inflammatory, Antioxidant, and Antinociceptive Effect In Vivo and Increase Cell Viability In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1150355
Modern Language Association (MLA)
Falcão, Tamires Rocha…[et al.]. Libidibia ferrea Fruit Crude Extract and Fractions Show Anti-Inflammatory, Antioxidant, and Antinociceptive Effect In Vivo and Increase Cell Viability In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1150355
American Medical Association (AMA)
Falcão, Tamires Rocha& Araújo, Aurigena Antunes de& Soares, Luiz Alberto Lira& Farias, Iuri Brilhante de& Silva, Wliana Alves Viturino da& Ferreira, Magda Rhayanny Assunção…[et al.]. Libidibia ferrea Fruit Crude Extract and Fractions Show Anti-Inflammatory, Antioxidant, and Antinociceptive Effect In Vivo and Increase Cell Viability In Vitro. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1150355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150355