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Development of a MD-LC-MSMS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats
Joint Authors
Cui, Yahua
Yang, Chang
Liang, Jun
Zhong, Linying
Liu, Caifeng
Bai, Jie
Du, Shouying
Liu, Daofang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Huoxuezhitong rubber patch, a well-known traditional Chinese medicine (TCM) prescription, is utilized to treat pain and inflammation.
In this study, a microdialysis-ultra-high-performance liquid chromatography-tandem mass spectrometry (MD-LC-MS/MS) method was designed for the simultaneous determination of active constituents in the rubber patch, such as paeonol (Pae), eugenol (Eug), and piperine (Pip).
A microdialysis probe was implanted in the subcutaneous tissue of a rat, which is intended to detect the subcutaneous concentrations of target components.
Saline containing 30% ethanol acted as perfusion fluid.
Analytes in the microdialysate were completely separated over an ACQITY UPLC RBEH C18 column (2.1mm×100mm, 1.7μm).
The mobile phase was composed of 0.01% ammonia aqueous and acetonitrile-0.01% ammonia with gradient elution.
The single-run analysis time was 10.0 minutes.
The linear regression displayed good linear relationships in the ranges of 0.25–100 ng/mL for paeonol and eugenol and 0.001–5 ng/mL for piperine.
The interday and intraday precision of the quality control samples exhibited relative standard deviations (RSD) <13.56%.
The accuracy values ranged from −14.92% to 14.00%.
The present method was successfully applied in pharmacokinetics studies following dermal administration of Huoxuezhitong rubber patch in rats.
Pip’s Tmax (488.00±150.73) min was greater than that of Pae (186.67±48.44) min and Eug (240.00±138.56) min, and the rank order of t1/2 was Pae > Pip > Eug.
The rank order of AUC0-720 and Cmax was both Eug > Pae > Pip.
MRT0-∞ of Pip was higher than that of Pae and Eug.
Eugenol showed a faster elimination and a shorter half-life.
Paeonol showed a stronger drug reservoir function after removing the drug source.
American Psychological Association (APA)
Cui, Yahua& Yang, Chang& Liang, Jun& Zhong, Linying& Liu, Caifeng& Bai, Jie…[et al.]. 2019. Development of a MD-LC-MSMS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1150388
Modern Language Association (MLA)
Cui, Yahua…[et al.]. Development of a MD-LC-MSMS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1150388
American Medical Association (AMA)
Cui, Yahua& Yang, Chang& Liang, Jun& Zhong, Linying& Liu, Caifeng& Bai, Jie…[et al.]. Development of a MD-LC-MSMS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1150388
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150388