Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil
Joint Authors
Aziz, Shujahadeen B.
Sha, Aram Mohammed
Mohammed, Sewara J.
Amin, Hassan H. H.
Hassan, Sarwar
Abdul Aziz, Jeza M.
Rahman, Heshu S.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study was aimed to investigate the structure of bioactive components of black seed oil (BSO) and their antimicrobial and cytotoxic effects.
Initially, the structural examination was conducted using various spectroscopic techniques, such as FTIR, TLC, and UV-visible spectroscopy, which are important in determining substituents, functional groups, and the presence of conjugated double bonds in BSO.
From the FTIR spectra, a variety of sharp, strong, and weak peaks were specified relating to the main components of thymoquinone (TQ), dithymoquinone, thymohydroquinone, and thymol in BSO.
The results of UV-visible spectroscopy confirmed the presence of thymoquinone as a major compound, and conjugated double bonds were also found.
In addition, qualitative TLC analysis was used to identify thymoquinone from the methanol-extracted layer in BSO, by calculating the retention factor (Rf) value.
Furthermore, antimicrobial activity of BSO was studied against various types of bacteria.
Strong bacterial inhibitory effects were observed, especially against Bacillus subtilis, with an average inhibition zone of 15.74 mm.
Moreover, through the use of the MTT assay in vitro, it was shown that BSO does not exhibit any cytotoxicity towards human peripheral blood mononuclear cells (PBMCs).
It was also found from the structural characterization of BSO that the existence of TQ is responsible for potential antibacterial activity without any cytotoxic effects.
The main observation of this work is that BSO has antimicrobial activity even against methicillin-resistant Staphylococcus aureus (MRSA).
American Psychological Association (APA)
Mohammed, Sewara J.& Amin, Hassan H. H.& Aziz, Shujahadeen B.& Sha, Aram Mohammed& Hassan, Sarwar& Abdul Aziz, Jeza M.…[et al.]. 2019. Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1150566
Modern Language Association (MLA)
Mohammed, Sewara J.…[et al.]. Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1150566
American Medical Association (AMA)
Mohammed, Sewara J.& Amin, Hassan H. H.& Aziz, Shujahadeen B.& Sha, Aram Mohammed& Hassan, Sarwar& Abdul Aziz, Jeza M.…[et al.]. Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1150566
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150566