Development of a HPLC-MSMS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats
Joint Authors
He, Jun
Ma, Wenjuan
Yao, Guangzhe
Jia, Qi
Ouyang, Huizi
Huang, Xuhua
Shen, Jiayuan
Zhang, Huamei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study developed a method for simultaneous determination of 13 elements of Semen Cuscutae (quercitrin, quercetin, hyperoside, caffeic acid, chlorogenic acid, luteolin, apigenin, kaempferol, isoquercitrin, cryptochlorogenic acid, isorhamnetin-3-O-glucoside, astragalin, and rutin) in rat plasma using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in the negative MRM mode.
The analytes were analyzed with CORTECS®C18 column (4.6 × 150 mm, 2.7 μm) with mobile phases consisting of 0.1% formic acid in water (A) and acetonitrile (B).
The intra- and interday precision of the target compounds were expressed as relative standard deviation (RSD) in the range of 0.5%–10.4%, and the accuracy of the target compounds was expressed as relative error (RE) not exceeding ±14.5% for all analytes.
In the meantime, the extraction recovery of the target compounds in plasma samples ranged from 87.4% to 106.2% and matrix effect from 81.0% to 115.5%.
The established method was successfully accomplished for the pharmacokinetic study of the analytes in rat plasma samples following oral administration of Semen Cuscutae extract, and the pharmacokinetic parameters of seven compounds were obtained.
American Psychological Association (APA)
Shen, Jiayuan& Jia, Qi& Huang, Xuhua& Yao, Guangzhe& Ma, Wenjuan& Zhang, Huamei…[et al.]. 2019. Development of a HPLC-MSMS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1150570
Modern Language Association (MLA)
Shen, Jiayuan…[et al.]. Development of a HPLC-MSMS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1150570
American Medical Association (AMA)
Shen, Jiayuan& Jia, Qi& Huang, Xuhua& Yao, Guangzhe& Ma, Wenjuan& Zhang, Huamei…[et al.]. Development of a HPLC-MSMS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1150570
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150570