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Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis
Joint Authors
Liu, Tian-Hao
Yang, Yong-hui
Zhang, Li-da
Chen, Zhu-yue
Huang, Xiao-shuang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Needle knife therapy, a form of acupuncture and moxibustion, has been widely used in the clinical treatment of knee osteoarthritis (KOA).
However, the mechanism is not clear.
Therefore, we studied the mechanisms of action of needle knife intervention on KOA in rabbits, with the PERK-eIF2α-CHOP pathway as a starting point, in order to determine the mechanism underlying knee joint chondrocyte apoptosis.
Apoptosis and ultrastructural changes in the articular cartilage were examined by pathological study and transmission electron microscopy, and PERK, eIF2α, and CHOP mRNA and protein levels were detected by qRT-PCR and western blot, respectively.
PERK, eIF2α, and CHOP protein levels were significantly higher in the model group than in the normal group (P < 0.01) and were considerably downregulated in the needle knife and the medicine groups compared to the model group (P < 0.01).
The eIF2α, p-eIF2α, and CHOP protein levels were not significantly different between the needle knife and medicine groups.
The PERK, eIF2α, and CHOP mRNA levels in the drug group were higher than those in the needle knife group (P < 0.01).
Needle knife therapy can regulate PERK-eIF2α-CHOP signaling pathway, which could be one of the mechanisms by which it affects chondrocyte apoptosis in KOA rabbits.
American Psychological Association (APA)
Yang, Yong-hui& Liu, Tian-Hao& Zhang, Li-da& Chen, Zhu-yue& Huang, Xiao-shuang. 2019. Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1150756
Modern Language Association (MLA)
Yang, Yong-hui…[et al.]. Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1150756
American Medical Association (AMA)
Yang, Yong-hui& Liu, Tian-Hao& Zhang, Li-da& Chen, Zhu-yue& Huang, Xiao-shuang. Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1150756
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150756