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Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis
Joint Authors
Xiong, Lize
Dong, Hailong
Bai, Fuhai
Ma, Yongyuan
Guo, Haiyun
Li, Yuheng
Xu, Feifei
Deng, Jiao
Zhang, Ming
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-07
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The concept of “acupoint sensitization” refers to the functional status of acupoint switches from silent to active under pathological conditions.
In clinic, acupoint sensitization provides important guidance for acupoints selection in different diseases.
However, the mechanism behind this phenomenon remains unclear.
We generated a model of knee osteoarthritis (KOA) by intra-articular injection of monosodium iodoacetate (MIA) into the left knee of rats.
The paw withdrawal mechanical threshold (PWMT) and the total number of mast cells as well as mast cell degranulation rate (MCDR) of acupoint tissue were used to test whether the acupoints were sensitized.
The results showed that KOA resulted in a reduced mechanical threshold and elevated total number of mast cell as well as mast cell degranulation rate at bilateral ST35 (Dubi) but not GB37 (Guangming) or nonacupoint area.
The acupoint sensitization was accompanied by upregulation of glycine transporter 2 (GlyT2) and reduction of extracellular glycine levels in the bilateral dorsal horns of the spinal cord at L3-5.
Selective inhibition of GlyT2 or intrathecal administration of glycine attenuated ST35 acupoint sensitization.
The sensitization of bilateral ST35 was blocked after intraspinal GlyT2 short hairpin (sh) RNA (GlyT2-shRNA) microinjection to specifically downregulate GlyT2 expression in the left side (ipsilateral) L3-5 spinal cord dorsal horn before MIA injection.
Moreover, electroacupuncture (EA) stimulation at ST35 ameliorated articular pathological lesions and improved KOA-related pain behaviors.
GlyT2-shRNA injection reversed EA-induced pain relief but not EA-induced reduction of joint lesions.
Overall, this study demonstrated that spinal GlyT2, especially elevated GlyT2 expression in the ipsilateral dorsal horn of the spinal cord, is a crucial mediator of ST35 acupoint sensitization in KOA rats.
American Psychological Association (APA)
Bai, Fuhai& Ma, Yongyuan& Guo, Haiyun& Li, Yuheng& Xu, Feifei& Zhang, Ming…[et al.]. 2019. Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1150892
Modern Language Association (MLA)
Bai, Fuhai…[et al.]. Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1150892
American Medical Association (AMA)
Bai, Fuhai& Ma, Yongyuan& Guo, Haiyun& Li, Yuheng& Xu, Feifei& Zhang, Ming…[et al.]. Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1150892
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150892