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Simiao Pill Attenuates Collagen-Induced Arthritis in Rats through Suppressing the ATX-LPA and MAPK Signalling Pathways
Joint Authors
Chen, Zhe
Wang, Hui
Shen, Pan
Qin, Kai
Tu, Shenghao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
Simiao pill (SM), a traditional Chinese formula, has been used as an antirheumatic drug in clinical practice for hundreds of years.
Rheumatoid arthritis (RA) is characterized by chronic synovial inflammation and hyperplasia, cartilage destruction, and joint damage.
This study was designed to investigate the protective effects of SM on collagen-induced arthritis (CIA) in rats.
It also aimed to explore whether this protective effect of SM was related to the inhibition of the ATX-LPA and MAPK signalling pathways.
Materials and Methods.
Rats were injected with a collagen II emulsion at the end of the tail and on the back to induce arthritis.
Treatment with different doses of SM was conducted by intragastric administration.
Then, body weights and arthritis scores were measured.
The serum levels of tumour necrosis factor (TNF)-α, interleukin (IL)-1β, C-reactive protein (CRP), osteoprotegerin (OPG), autotaxin (ATX), and lysophosphatidic acid (LPA) were determined by ELISA.
Pathological changes in the joints were measured by micro-CT and assessed via haematoxylin-eosin (H&E) staining.
The expression of ATX, LPA receptor 1 (LPA1) was detected by immunohistochemical staining, and the expression of mitogen-activated protein kinase (MAPK) was detected by Western blotting.
Results.
SM significantly alleviated arthritis symptoms, inhibited bone erosion, and decreased the levels of TNF-α, IL-1β, CRP, ATX, and LPA in the sera of CIA rats.
Importantly, SM clearly reduced the protein expression of LPA1 and ATX.
The activation of the MAPK signalling pathway was also inhibited by SM in the synovial tissues of CIA rats.
Conclusions.
The antirheumatic effects of SM were associated with the regulation of the ATX-LPA and MAPK pathways, the suppression of proinflammatory cytokine production, and the alleviation of cartilage and bone injury.
These findings suggest that SM might be a promising alternative candidate for RA therapy.
American Psychological Association (APA)
Shen, Pan& Tu, Shenghao& Wang, Hui& Qin, Kai& Chen, Zhe. 2019. Simiao Pill Attenuates Collagen-Induced Arthritis in Rats through Suppressing the ATX-LPA and MAPK Signalling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1150905
Modern Language Association (MLA)
Shen, Pan…[et al.]. Simiao Pill Attenuates Collagen-Induced Arthritis in Rats through Suppressing the ATX-LPA and MAPK Signalling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1150905
American Medical Association (AMA)
Shen, Pan& Tu, Shenghao& Wang, Hui& Qin, Kai& Chen, Zhe. Simiao Pill Attenuates Collagen-Induced Arthritis in Rats through Suppressing the ATX-LPA and MAPK Signalling Pathways. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1150905
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150905