Network Pharmacology and Bioinformatics Approach Reveals the Therapeutic Mechanism of Action of Baicalein in Hepatocellular Carcinoma
Joint Authors
Wei, Wei
Cheng, Fafeng
Wang, Xueqian
Ma, Chongyang
Zhai, Changming
Xu, Tian
Zhang, Shuang
Li, Changxiang
Fan, Shuning
Liu, Shuling
Lei, Chaofang
Tang, Feifei
Luo, Juan
Sun, Xiaoguang
Wang, Qingguo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-12
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Liver cancer is the fourth leading cause of cancer death worldwide, and hepatocellular carcinoma (HCC) accounts for the greatest proportion of these deaths.
Baicalein, a flavonoid isolated from the root of Scutellariae radix, is considered a potential candidate to treat HCC.
However, the underlying molecular mechanisms remain poorly understood.
In the present study, a network pharmacological approach was combined with microarray data (GSE95504) acquired from the Gene Expression Omnibus database to reveal the therapeutic mechanisms of action of baicalein at a systemic level.
We identified 38 baicalein targets and 76 differently expressed genes (DEGs) following treatment with baicalein, including 55 upregulated and 21 downregulated genes.
The DEGs were significantly enriched in the biological functions of apoptosis, endoplasmic reticulum stress, and PERK-mediated unfolded protein response.
Protein-protein interaction (PPI) network construction and topological screening revealed a core module of PPIs including two baicalein targets, TP53 and CDK1, and two downregulated DEGs, HSPA1A and HSPA1B.
Expression and survival data for these genes in the module derived from Gene Expression Profiling Interactive Analysis (GEPIA) were subjected to Kaplan–Meier analysis of overall survival and disease-free survival.
Overexpression of CDK1, BRCA1, TUBB, HSPA1A, HSPA1B, and HSPA4 was associated with significantly worse overall survival, while overexpression of CDK1, CLU7, BRCA1, and TUBB was associated with significantly worse disease-free survival.
These data suggest that baicalein exerts therapeutic effects against HCC via a PPI network involving TP53, CDK1, HSPA1A, and HSPA1B.
American Psychological Association (APA)
Ma, Chongyang& Xu, Tian& Sun, Xiaoguang& Zhang, Shuang& Liu, Shuling& Fan, Shuning…[et al.]. 2019. Network Pharmacology and Bioinformatics Approach Reveals the Therapeutic Mechanism of Action of Baicalein in Hepatocellular Carcinoma. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1150934
Modern Language Association (MLA)
Ma, Chongyang…[et al.]. Network Pharmacology and Bioinformatics Approach Reveals the Therapeutic Mechanism of Action of Baicalein in Hepatocellular Carcinoma. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1150934
American Medical Association (AMA)
Ma, Chongyang& Xu, Tian& Sun, Xiaoguang& Zhang, Shuang& Liu, Shuling& Fan, Shuning…[et al.]. Network Pharmacology and Bioinformatics Approach Reveals the Therapeutic Mechanism of Action of Baicalein in Hepatocellular Carcinoma. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1150934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150934