Stingless Bee Honey Improves Spatial Memory in Mice, Probably Associated with Brain-Derived Neurotrophic Factor (BDNF) and Inositol 1,4,5-Triphosphate Receptor Type 1 (Itpr1) Genes
Joint Authors
Mustafa, Mohd Zulkifli
Zulkifli, Fairuz Nabila
Fernandez, Ivanna
Mariatulqabtiah, Abdul Razak
Sangu, Muthuraju
Nor Azfa, Johari
Roslan, Nurhidayah
Mohamed, Mahaneem
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study was conducted to evaluate the effects of stingless bee honey (SBH) supplementation on memory and learning in mice.
Despite many studies that show the benefits of honey on memory, reports on the nootropic effects of SBH are still lacking, and their underlying mechanism is still unclear.
SBH is a honey produced by the bees in the tribe of Meliponini that exist in tropical countries.
It features unique storage of honey collected in cerumen pots made of propolis.
This SBH may offer a better prospect for therapeutic performance as the previous report identifies the presence of antioxidants that were greater than other honey produced by Apis sp.
In this study, SBH was tested on Swiss albino mice following acute (7 days) and semichronic (35 days) supplementation.
Experiments were then conducted using Morris water maze (MWM) behaviour analysis, RT-PCR for gene expression of mice striatum, and NMR for metabolomics analysis of the honey.
Results indicate spatial working memory and spatial reference memory of mice were significantly improved in the honey-treated group compared with the control group.
Improved memory consolidations were also observed in prolonged supplementation.
Gene expression analyses of acutely treated mice demonstrated significant upregulation of BDNF and Itpr1 genes that involve in synaptic function.
NMR analysis also identified phenylalanine, an essential precursor for tyrosine that plays a role at the BDNF receptor.
In conclusion, SBH supplementation for seven days at 2000 mg/kg, which is equivalent to a human dose of 162 mg/kg, showed strong capabilities to improve spatial working memory.
And prolonged intake up to 35 days increased spatial reference memory in the mice model.
The phenylalanine in SBH may have triggered the upregulation of BDNF genes in honey-treated mice and improved their spatial memory performance.
American Psychological Association (APA)
Mustafa, Mohd Zulkifli& Zulkifli, Fairuz Nabila& Fernandez, Ivanna& Mariatulqabtiah, Abdul Razak& Sangu, Muthuraju& Nor Azfa, Johari…[et al.]. 2019. Stingless Bee Honey Improves Spatial Memory in Mice, Probably Associated with Brain-Derived Neurotrophic Factor (BDNF) and Inositol 1,4,5-Triphosphate Receptor Type 1 (Itpr1) Genes. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1151150
Modern Language Association (MLA)
Mustafa, Mohd Zulkifli…[et al.]. Stingless Bee Honey Improves Spatial Memory in Mice, Probably Associated with Brain-Derived Neurotrophic Factor (BDNF) and Inositol 1,4,5-Triphosphate Receptor Type 1 (Itpr1) Genes. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1151150
American Medical Association (AMA)
Mustafa, Mohd Zulkifli& Zulkifli, Fairuz Nabila& Fernandez, Ivanna& Mariatulqabtiah, Abdul Razak& Sangu, Muthuraju& Nor Azfa, Johari…[et al.]. Stingless Bee Honey Improves Spatial Memory in Mice, Probably Associated with Brain-Derived Neurotrophic Factor (BDNF) and Inositol 1,4,5-Triphosphate Receptor Type 1 (Itpr1) Genes. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1151150
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151150