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The Modified Bushen Antai Recipe Upregulates Estrogen and Progesterone Receptors at the Maternal-Fetal Interface in Pregnant Rats with Mifepristone-Induced Pregnancy Loss
Joint Authors
Sun, Li
Yuan, Zhengwei
Jian, Lingyan
Jiang, Qinghua
Zhang, Siwen
Tan, Jichun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
The modified Bushen Antai recipe (BSAT) is a centuries-old traditional Chinese medicine that we use in our center as a therapy against pregnancy loss.
Our study aimed to explore the potential benefit and mechanism of BSAT in pregnant rats with mifepristone-induced pregnancy loss.
Materials and Methods.
The signature compounds of the eight BSAT ingredients were analyzed by high-performance liquid chromatography (HPLC).
The BSAT group (n = 8) was treated daily with 6.3 ml/kg BSAT from gestation day (D) 0.5 to 10.5 and once with 1.25 mg/kg mifepristone on D 10.5.
Normal saline replaced BSAT in the model group (n = 8), and both BSAT and mifepristone in the control group (n = 8).
Morphological and histological analyses were performed on D 13.5.
Results.
BSAT contains eight medicinal ingredients including Cuscuta chinensis and Dipsacus asperoides.
The HPLC analysis detected the signature compounds of seven medicinal ingredients in the extract.
Embryo resorption rate in the BSAT group was significantly lower than that in the model group, although the number of surviving embryos was similar between the two groups.
Hematoxylin and eosin (HE) staining suggested that the maximum cross-sectional area of the placenta and the area ratio of the placental labyrinth in the BSAT group were higher than those in the model group.
Immunohistochemical (IHC) staining indicated that the expression of ki67, estrogen receptor alpha (ERα), and progesterone receptor (PR) in the placental labyrinth of the BSAT group was higher than that of the model group.
Furthermore, the protein levels of ERα, PR, phospho-Akt/Akt, and phospho-Erk1/2/Erk1/2 in the BSAT group were higher than those in the control group.
The mRNA levels of ERα and PR in the BSAT group were higher than those in the control group.
Conclusions.
BSAT may induce estrogen and progesterone receptors by phosphorylation via the classic Akt and Erk1/2 signaling pathways in the maternal-fetal interface of pregnant rats, thereby reducing the pregnancy loss rate and improving the live birth rate.
American Psychological Association (APA)
Sun, Li& Yuan, Zhengwei& Jian, Lingyan& Jiang, Qinghua& Zhang, Siwen& Tan, Jichun. 2019. The Modified Bushen Antai Recipe Upregulates Estrogen and Progesterone Receptors at the Maternal-Fetal Interface in Pregnant Rats with Mifepristone-Induced Pregnancy Loss. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1151198
Modern Language Association (MLA)
Sun, Li…[et al.]. The Modified Bushen Antai Recipe Upregulates Estrogen and Progesterone Receptors at the Maternal-Fetal Interface in Pregnant Rats with Mifepristone-Induced Pregnancy Loss. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1151198
American Medical Association (AMA)
Sun, Li& Yuan, Zhengwei& Jian, Lingyan& Jiang, Qinghua& Zhang, Siwen& Tan, Jichun. The Modified Bushen Antai Recipe Upregulates Estrogen and Progesterone Receptors at the Maternal-Fetal Interface in Pregnant Rats with Mifepristone-Induced Pregnancy Loss. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1151198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151198