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Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MSMS and Application of the Technique to Pharmacokinetic Analyses
Joint Authors
Gong, Zipeng
Li, YueTing
Huang, Yong
Zheng, Lin
Wang, Yong-Lin
Wang, Ai-Min
Li, Yong-Jun
Chen, Hao
Mei, Chao-Ye
Chen, Si-Ying
Lan, Yan-Yu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Bletilla striata has been widely used as a valuable hemostatic agent for thousands of years due to the high levels of bioactive constituents it contains.
Here, we used a sensitive ultrahigh-performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) method for the simultaneous determination of three major active ingredients of the B.
striata extract, namely, α-isobutylmalic acid, gymnoside I, and militarine in rat plasma.
The three major active ingredients were determined using the multiple reaction monitoring (MRM) mode at m/z 189 ⟶ 129 for α-isobutylmalic acid, m/z 457.2 ⟶ 285.1 for gymnoside I, m/z 725.3 ⟶ 457.2 for militarine, and m/z 417.0 ⟶ 267.0 for the IS puerarin.
All calibration curves showed good linearity (R2 ≥ 0.999) over the concentration range with the lower limit of quantification between 0.015 and 0.029 μg/mL.
The relative standard deviations of intraday and interday measurements were less than 15%, and the method was accurate within 93.3–100.4%.
The extraction recovery was 92.65–100.98%, and no matrix effect was observed.
The results indicated that after oral administration of B.
striata in rats, the Tmax of α-isobutylmalic acid was significantly longer than that of gymnoside I and militarine and the mean residence time and area under the curve of α-isobutylmalic acid and gymnoside I in rats were significantly higher than those of militarine.
Moreover, the blood concentration-time curve of α-isobutylmalic acid showed double peaks, suggesting that α-isobutylmalic acid could exhibit the phenomenon of enterohepatic circulation or metabolic conversion.
We also explored some of the pharmacokinetic characteristics of three ingredients from B.
striata extract in vivo, and the data obtained may provide a basis for the further investigation of B.
striata.
American Psychological Association (APA)
Chen, Hao& Zheng, Lin& Mei, Chao-Ye& Gong, Zipeng& Li, Yong-Jun& Chen, Si-Ying…[et al.]. 2019. Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MSMS and Application of the Technique to Pharmacokinetic Analyses. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151495
Modern Language Association (MLA)
Chen, Hao…[et al.]. Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MSMS and Application of the Technique to Pharmacokinetic Analyses. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1151495
American Medical Association (AMA)
Chen, Hao& Zheng, Lin& Mei, Chao-Ye& Gong, Zipeng& Li, Yong-Jun& Chen, Si-Ying…[et al.]. Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MSMS and Application of the Technique to Pharmacokinetic Analyses. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151495
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151495