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Adenine Inhibits the Growth of Colon Cancer Cells via AMP-Activated Protein Kinase Mediated Autophagy
Joint Authors
Wei, James Cheng-Chung
Lai, Hsin-Wu
Hung, Hung-Chang
Lin, Chun-Che
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Adenine is involved in a variety of cell biological processes and has been explored for pharmacological uses.
Its therapeutic use for managing cancer is of great interest.
In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells.
Methods.
Cell viability was measured using the MTT assay.
Levels of phosphorylation and protein expression were determined using western blotting.
qPCR was carried out to determine the changes in mRNA expression of genes of interest.
Results.
Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner.
Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses.
The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines.
Conclusions.
Our findings suggest that adenine inhibits the growth of colon cancer cells.
Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway.
Adenine represents a natural compound with anticancer potency.
American Psychological Association (APA)
Lai, Hsin-Wu& Wei, James Cheng-Chung& Hung, Hung-Chang& Lin, Chun-Che. 2019. Adenine Inhibits the Growth of Colon Cancer Cells via AMP-Activated Protein Kinase Mediated Autophagy. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1151598
Modern Language Association (MLA)
Lai, Hsin-Wu…[et al.]. Adenine Inhibits the Growth of Colon Cancer Cells via AMP-Activated Protein Kinase Mediated Autophagy. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1151598
American Medical Association (AMA)
Lai, Hsin-Wu& Wei, James Cheng-Chung& Hung, Hung-Chang& Lin, Chun-Che. Adenine Inhibits the Growth of Colon Cancer Cells via AMP-Activated Protein Kinase Mediated Autophagy. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1151598
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151598