Effect of Tripterygium wilfordii Polycoride on the NOXs-ROS-NLRP3 Inflammasome Signaling Pathway in Mice with Ulcerative Colitis

Joint Authors

Fangxiao, Ma
Yifan, Ke
Jihong, Zhong
Yan, Shen
Yingchao, Liu

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-08-21

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Medicine

Abstract EN

Objective.

To explore the effect of Tripterygium wilfordii polycoride (TWP) on the NADPH oxidases (NOXs)-reactive oxygen species (ROS)-NOD-like receptor protein 3 (NLRP3) inflammasome signaling pathway and the possibility of using TWP to treat ulcerative colitis (UC).

Methods.

BALB/c mice were randomly divided into five groups: model control, low TWP, middle TWP, high TWP, and normal control groups.

A UC model was established with dextran sulfate sodium.

The determination of ROS was carried out by using the fluorescent probe DCFH-DA, and NOXs activity was detected based on the NADPH consumption rate.

The mRNA expression levels of NLRP3, ASC, and caspase-1 in the colon tissues and neutrophils were assessed via real-time PCR.

Results.

The colon tissues were abnormal with different degrees in TWP groups with disease activity index and histopathological scores lower than those in the model group.

In TWP groups, ROS generation, NOXs activity, and the mRNA expression levels of NLRP3, ASC, and caspase-1 in the colon tissues and colon-isolated neutrophils were remarkably lower than those in the model control group (P<0.05) and higher than those in the normal group (P<0.05).

The results of pairwise comparison for the efficacy of TWP administration showed that the above indexes were statistically significant with the lowest expression in the high TWP group (P<0.05) and the highest expression in the low TWP group (P<0.05).

Conclusion.

TWP demonstrated anti-inflammatory effects on UC by decreasing the expression of proinflammatory factors in the NOXs-ROS-NLRP3 signaling pathway.

American Psychological Association (APA)

Fangxiao, Ma& Yifan, Ke& Jihong, Zhong& Yan, Shen& Yingchao, Liu. 2019. Effect of Tripterygium wilfordii Polycoride on the NOXs-ROS-NLRP3 Inflammasome Signaling Pathway in Mice with Ulcerative Colitis. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1151654

Modern Language Association (MLA)

Fangxiao, Ma…[et al.]. Effect of Tripterygium wilfordii Polycoride on the NOXs-ROS-NLRP3 Inflammasome Signaling Pathway in Mice with Ulcerative Colitis. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1151654

American Medical Association (AMA)

Fangxiao, Ma& Yifan, Ke& Jihong, Zhong& Yan, Shen& Yingchao, Liu. Effect of Tripterygium wilfordii Polycoride on the NOXs-ROS-NLRP3 Inflammasome Signaling Pathway in Mice with Ulcerative Colitis. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1151654

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1151654