Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves
Joint Authors
Wang, Yi-Ting
Huang, Ting-Yu
Tai, Mi‐Hsueh
Sie, Ru-Han
Shaw, Jei-Fu
Yang, Chih-Hui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Chlorophyllide (chlide) is a natural catabolic product of chlorophyll (Chl), produced through the activity of chlorophyllase (chlase).
The growth inhibitory and antioxidant effects of chlide from different plant leaf extracts have not been reported.
The aim of this study is to demonstrate that chlide in crude extracts from leaves has the potential to exert cytotoxic effects on cancer cell lines.
The potential inhibitory and antioxidant effects of chlide in crude extracts from 10 plant leaves on breast cancer cells (MCF7 and MDA-MB-231), hepatocellular carcinoma cells (Hep G2), colorectal adenocarcinoma cells (Caco2), and glioblastoma cells (U-118 MG) were studied using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays.
The results of the MTT assay showed that chlide in crude extracts from sweet potato were the most effective against all cancer cell lines tested.
U-118 MG cells were the most sensitive, while Caco2 cells were the most resistant to the tested crude extracts.
The cytotoxic effects of chlide and Chl in crude extracts from sweet potato and of commercial chlorophyllin (Cu-chlin), in descending order, were as follows: chlide > Chl > Cu-chlin.
Notably, the IC50 of sweet potato in U-118 MG cells was 45.65 μg/mL while those of Chl and Cu-chlin exceeded 200 μg/mL.
In the DPPH assay, low concentrations (100 μg/mL) of chlide and Cu-chlin from crude extracts of sweet potato presented very similar radical scavenging activity to vitamin B2.
The concentration of chlide was negatively correlated with DPPH activity.
The current study was the first to demonstrate that chlide in crude extracts from leaves have potential cytotoxicity in cancer cell lines.
Synergism between chlide and other compounds from leaf crude extracts may contribute to its cytotoxicity.
American Psychological Association (APA)
Wang, Yi-Ting& Yang, Chih-Hui& Huang, Ting-Yu& Tai, Mi‐Hsueh& Sie, Ru-Han& Shaw, Jei-Fu. 2019. Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1151736
Modern Language Association (MLA)
Wang, Yi-Ting…[et al.]. Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1151736
American Medical Association (AMA)
Wang, Yi-Ting& Yang, Chih-Hui& Huang, Ting-Yu& Tai, Mi‐Hsueh& Sie, Ru-Han& Shaw, Jei-Fu. Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1151736
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151736