Effects of Crinum jagus WaterEthanol Extract on Shigella flexneri-Induced Diarrhea in Rats
Joint Authors
Wambe, Henri
Noubissi, Paul Aimé
Fokam Tagne, Michel Archange
Fankem, Gaëtan Olivier
Ngakou Mukam, Joseph
Kamgang, René
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Diarrheal disease, characterized by the release of more than three loose or liquid stools per day, remains one of the leading causes of morbidity and mortality in children below 5 years of age in developing countries.
Many drugs used in diarrhea management face contraindication and, with regard to infectious diarrhea, resistance of some bacterial strains; this therefore increases the need of new alternative and more effective drugs.
This study aimed to evaluate anti-Shigella flexneri activities of Crinum jagus water/ethanol extract.
In vitro activities were assayed by disc diffusion and agar dilution methods and in vivo section on Shigella flexneri-induced diarrhea in rats.
This was done by oral administration of 9 X 108 CFU of Shigella flexneri to rats that were treated twice daily with Crinum jagus water/ethanol extract for seven consecutive days.
Ciprofloxacin was used as positive control.
Daily Shigella flexneri load was evaluated.
After one treatment week, animals were then sacrificed and interleukins (IL-2 and INF-γ), immunoglobulins (IgA and IgM), motilin, vasoactive intestinal peptide, and ions (sodium, potassium, calcium, and chloride) levels were determined.
Also, blood cell count was realized.
Crinum jagus water/ethanol extract dose-dependently inhibited Shigella flexneri growth with inhibition diameter of 18.90 and 25.36 mm, respectively, at 0.39 and 200 mg/mL.
Minimum inhibitory concentration (MIC) was 0.10 mg/mL and minimum bactericidal concentration (MBC) was 0.30 mg/mL with MBC/MIC ratio of 3.0.
In Shigella flexneri-induced diarrheic rats, Crinum jagus reduced (p<0.01) diarrheal stools emission and Shigella load and lowered IL-2, INF-γ, IgA, IgM, and motilin blood levels, whereas it increased (p<0.01) vasoactive intestinal peptide, sodium, potassium, calcium, and chloride blood levels.
In diarrheal rats, Crinum jagus restored the decreasing white blood cells and haemoglobin and restored the damaged colon epithelium, where it reduced the density of mucus-filled goblet cells.
These results confirm the use of Crinum jagus in ethnomedicine in diarrhea treatment.
American Psychological Association (APA)
Noubissi, Paul Aimé& Fokam Tagne, Michel Archange& Fankem, Gaëtan Olivier& Ngakou Mukam, Joseph& Wambe, Henri& Kamgang, René. 2019. Effects of Crinum jagus WaterEthanol Extract on Shigella flexneri-Induced Diarrhea in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151748
Modern Language Association (MLA)
Noubissi, Paul Aimé…[et al.]. Effects of Crinum jagus WaterEthanol Extract on Shigella flexneri-Induced Diarrhea in Rats. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1151748
American Medical Association (AMA)
Noubissi, Paul Aimé& Fokam Tagne, Michel Archange& Fankem, Gaëtan Olivier& Ngakou Mukam, Joseph& Wambe, Henri& Kamgang, René. Effects of Crinum jagus WaterEthanol Extract on Shigella flexneri-Induced Diarrhea in Rats. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151748
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151748