MIP-T3 Expression Associated with Defects of Ciliogenesis in Airway of COPD Patients
Joint Authors
Wang, Wen-Jun
Yang, Shi-Fang
Gao, Zhi-Rui
Luo, Ze-Ru
Liu, Yuan-Ling
Gao, Xing-Lin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Introduction.
Some studies have found that cilia were shorter in COPD smokers than in nonsmokers or healthy smokers.
However, the structural abnormalities of cilia and the cause of such abnormalities in COPD patients still remain unknown.
Tumor necrosis factor alpha receptor 3 interacting protein 1 (MIP-T3) may play an important role in the progress of ciliary protein transporting.
Objectives.
This study aimed at exploring the dominated structural abnormalities of cilia and the involvement of MIP-T3 in the pathogenesis of cilia of COPD patients.
Methods.
Patients who accepted pulmonary lobectomy were divided into 3 groups: the chronic obstructive pulmonary disease (COPD) smoker group, the healthy smoker group, and the nonsmoker group, according to smoking history and pulmonary function.
The ultrastructure of cilia and the percentage of abnormal cilia were analyzed using a transmission electron microscope.
Real-time PCR, immunohistochemical staining, and western blotting in bronchial epithelium were used to determine MIP-T3 mRNA and protein expression.
The relationship between the percentage of abnormal cilia and lung function and MIP-T3 protein expression was analyzed.
Results.
Patients in the COPD smoker group had increased percentage of abnormal cilia comparing to both the healthy smoker group and the nonsmoker group (both P values <0.05).
MIP-T3 expression was significantly declined in the COPD smoker group (P value <0.05).
Moreover, the percentage of abnormal cilia was negatively correlated with FEV1, FEV1/FVC ratio, and FEV1%pred (all P values <0.05).
Moreover, the MIP-T3 protein expression was positively correlated with the percentage of abnormal cilia (P value <0.05).
Conclusions.
Our results suggested that the abnormal ciliary ultrastructure, which was common in COPD patients, might be due to MIP-T3 downregulation.
American Psychological Association (APA)
Wang, Wen-Jun& Yang, Shi-Fang& Gao, Zhi-Rui& Luo, Ze-Ru& Liu, Yuan-Ling& Gao, Xing-Lin. 2020. MIP-T3 Expression Associated with Defects of Ciliogenesis in Airway of COPD Patients. Canadian Respiratory Journal،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1152084
Modern Language Association (MLA)
Wang, Wen-Jun…[et al.]. MIP-T3 Expression Associated with Defects of Ciliogenesis in Airway of COPD Patients. Canadian Respiratory Journal No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1152084
American Medical Association (AMA)
Wang, Wen-Jun& Yang, Shi-Fang& Gao, Zhi-Rui& Luo, Ze-Ru& Liu, Yuan-Ling& Gao, Xing-Lin. MIP-T3 Expression Associated with Defects of Ciliogenesis in Airway of COPD Patients. Canadian Respiratory Journal. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1152084
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152084