Diagnostic Value of the Methylation of Multiple Gene Promoters in Serum in Hepatitis B Virus-Related Hepatocellular Carcinoma
Joint Authors
Dong, Xueyan
Hou, Qiang
Chen, Yueming
Wang, Xianjun
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-29
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This study sought to evaluate the diagnostic value of the methylation of multiple gene promoters in serum in hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC).
A total of 343 participants were enrolled, including 98 patients with HCC, 75 patients with liver cirrhosis (LC), 90 patients with chronic hepatitis B (CHB), and 80 healthy individuals.
RASSF1A, APC, BVES, TIMP3, GSTP1, and HOXA9 were selected as the candidate genes.
The MethyLight method was used to assay promoter methylation statuses.
The diagnostic performances of markers were assessed by constructing receiver operating characteristic (ROC) curves.
The prevalences of methylation for RASSF1A, APC, BVES, HOXA9, GSTP1, and TIMP3 were 52.04%, 36.73%, 29.59%, 20.41%, 17.35%, and 11.22%, respectively.
APC methylation completely overlapped with RASSF1A methylation.
The area under the curve (AUC) for RASSF1A methylation (0.718) was better than the corresponding AUC for AFP (0.609) in distinguishing HCC from CHB.
When RASSF1A, BVES, HOXA9, and AFP were combined, the AUC was 0.852 (95% CI = 0.796–0.908, P=0.028), and the sensitivity and specificity were 83.7% and 78.9%, respectively.
In conclusion, an assay that combines methylation of the RASSF1A, BVES, and HOXA9 gene promoters in serum and AFP could significantly improve HBV-related HCC diagnoses.
American Psychological Association (APA)
Dong, Xueyan& Hou, Qiang& Chen, Yueming& Wang, Xianjun. 2017. Diagnostic Value of the Methylation of Multiple Gene Promoters in Serum in Hepatitis B Virus-Related Hepatocellular Carcinoma. Disease Markers،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1152264
Modern Language Association (MLA)
Dong, Xueyan…[et al.]. Diagnostic Value of the Methylation of Multiple Gene Promoters in Serum in Hepatitis B Virus-Related Hepatocellular Carcinoma. Disease Markers No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1152264
American Medical Association (AMA)
Dong, Xueyan& Hou, Qiang& Chen, Yueming& Wang, Xianjun. Diagnostic Value of the Methylation of Multiple Gene Promoters in Serum in Hepatitis B Virus-Related Hepatocellular Carcinoma. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1152264
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152264