Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods
Joint Authors
Daidone, Maria Grazia
Fina, Emanuela
Necchi, Andrea
Bottelli, Stefano
Reduzzi, Carolina
Pizzamiglio, Sara
Iacona, Chiara
Verderio, Paolo
Cappelletti, Vera
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Circulating tumour cells (CTC) are identified exploiting their protein/gene expression patterns or distinct size compared to blood cells.
Data on CTC in bladder cancer (BC) are still scarce.
We comparatively analyzed CTC enrichment by AdnaTest ProstateCancerSelect (AT) and ScreenCell®Cyto (SC) kits, combined with identification by EPCAM, MUC1, and ERBB2 expression and by cytological criteria, respectively, in 19 nonmetastatic (M0) and 47 metastatic (M+) BC patients, at baseline (T0) and during treatment (T1).
At T0, CTC positivity rates by AT were higher in M+ compared to M0 cases (57.4% versus 25%, p = 0.041).
EPCAM was detected in 75% of CTC-positive samples by AT, showing increasing expression levels from T0 to T1 (median (interquartile range, IQR): 0.18 (0.07–0.42) versus 0.84 (0.33–1.84), p=0.005) in M+ cases.
Overall, CTC positivity by SC was around 80% regardless of clinical setting and time point of analysis, except for a lower occurrence at T1 in M0 cases.
At T0, circulating tumour microemboli were more frequently (25% versus 8%) detected and more numerous in M+ compared to M0 patients.
The approach used for CTC detection impacts the outcome of CTC studies.
Further investigations are required to clarify the clinical validity of AT and SC in specific BC clinical contexts.
American Psychological Association (APA)
Fina, Emanuela& Necchi, Andrea& Bottelli, Stefano& Reduzzi, Carolina& Pizzamiglio, Sara& Iacona, Chiara…[et al.]. 2017. Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods. Disease Markers،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152286
Modern Language Association (MLA)
Fina, Emanuela…[et al.]. Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods. Disease Markers No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1152286
American Medical Association (AMA)
Fina, Emanuela& Necchi, Andrea& Bottelli, Stefano& Reduzzi, Carolina& Pizzamiglio, Sara& Iacona, Chiara…[et al.]. Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152286