Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms
Joint Authors
Hernández-Navarro, María Dolores
Mociño-Rodríguez, Martha Daniela
Santillán-Benítez, Jonnathan Guadalupe
Dozal-Domínguez, David Salomón
Flores-Merino, Miriam Veronica
Sandoval-Cabrera, Antonio
García Vázquez, Francisco Javier
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The development of breast cancer is influenced by the adipose tissue through the proteins leptin and adiponectin.
However, there is little research concerning AdipoR1 and AdipoR2 receptors and the influence of leptin over them.
The objective of this work was to analyze the expression of AdipoR1 and AdipoR2, modulated by differential concentrations of leptin in an obesity model (10 ng/mL, 100 ng/mL, and 1000 ng/mL) associated with breast cancer in MCF-7 and HCC1937 cell lines.
Each cell line was characterized through immunohistochemistry, and the expression of AdipoR1 and AdipoR2 was analyzed by PCR in real time using TaqMan® probes.
Leptin induced an increase in cell population of MCF-7 (23.8%, 10 ng/mL, 48 h) and HCC1937 (17.24%, 1000 ng/mL, 72 h).
In MCF-7, the expression of AdipoR1 decreased (3.81%, 1000 ng/mL) and the expression of AdipoR2 increased by 13.74 times (10 ng/mL) with regard to the control.
In HCC1937, the expression of AdipoR1 decreased by 86.28% (10 ng/mL), as well as the expression of AdipoR2 (50.3%, 100 ng/mL).
In regard to the results obtained, it could be concluded that leptin has an effect over the expression of AdipoR1 and AdipoR2 mRNA.
American Psychological Association (APA)
Mociño-Rodríguez, Martha Daniela& Santillán-Benítez, Jonnathan Guadalupe& Dozal-Domínguez, David Salomón& Hernández-Navarro, María Dolores& Flores-Merino, Miriam Veronica& Sandoval-Cabrera, Antonio…[et al.]. 2017. Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms. Disease Markers،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152353
Modern Language Association (MLA)
Mociño-Rodríguez, Martha Daniela…[et al.]. Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms. Disease Markers No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1152353
American Medical Association (AMA)
Mociño-Rodríguez, Martha Daniela& Santillán-Benítez, Jonnathan Guadalupe& Dozal-Domínguez, David Salomón& Hernández-Navarro, María Dolores& Flores-Merino, Miriam Veronica& Sandoval-Cabrera, Antonio…[et al.]. Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152353
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152353