LncRNA NEAT1 Regulates Cell Viability and Invasion in Esophageal Squamous Cell Carcinoma through the miR-129CTBP2 Axis

Joint Authors

Li, Yong
Chen, Dong
Gao, Xiang
Li, Xiaohui
Shi, Gongning

Source

Disease Markers

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-09-25

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Diseases

Abstract EN

Background.

Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) was reported to be aberrantly upregulated and promote esophageal squamous cell carcinoma (ESCC) cell progression.

Nevertheless, the molecular mechanism of NEAT1 involved in the competing endogenous RNA (ceRNA) regulatory network in ESCC progression remains poorly defined.

Methods.

The expressions of NEAT1, miR-129, and C-terminal-binding protein 2 (CTBP2) in ESCC cells were examined by qRT-PCR.

The effects of NEAT1 knockdown and miR-129 overexpression, or along with CTBP2 upregulation, on ESCC cell viability and invasion were explored by CCK-8 and transwell invasion assays, respectively.

Luciferase reporter assay in combination with RIP was performed to confirm the interaction between NEAT1, miR-129, and CTBP2.

Results.

NEAT1 and CTBP2 were upregulated and miR-129 was downregulated in ESCC cells.

Either NEAT1 knockdown or miR-129 overexpression suppressed ESCC cell viability and invasion.

Moreover, NEAT1 functioned as an endogenous sponge to downregulate miR-129 by competitively binding to miR-129, thereby leading to the derepression of CTBP2, a target of miR-129.

CTBP2 restoration overturned cell viability and invasion suppression mediated by NEAT1 knockdown or miR-129 overexpression.

Conclusion.

LncRNA NEAT1 regulated ESCC cell viability and invasion via the miR-129/CTBP2 axis, contributing to the better understanding of the molecular mechanism of ESCC pathogenesis and progression.

American Psychological Association (APA)

Li, Yong& Chen, Dong& Gao, Xiang& Li, Xiaohui& Shi, Gongning. 2017. LncRNA NEAT1 Regulates Cell Viability and Invasion in Esophageal Squamous Cell Carcinoma through the miR-129CTBP2 Axis. Disease Markers،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152382

Modern Language Association (MLA)

Li, Yong…[et al.]. LncRNA NEAT1 Regulates Cell Viability and Invasion in Esophageal Squamous Cell Carcinoma through the miR-129CTBP2 Axis. Disease Markers No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1152382

American Medical Association (AMA)

Li, Yong& Chen, Dong& Gao, Xiang& Li, Xiaohui& Shi, Gongning. LncRNA NEAT1 Regulates Cell Viability and Invasion in Esophageal Squamous Cell Carcinoma through the miR-129CTBP2 Axis. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152382

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1152382