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Upregulation of MicroRNA-125b Leads to the Resistance to Inflammatory Injury in Endothelial Progenitor Cells
Joint Authors
Yang, Ke
Liu, Xing
Lin, Wanwen
Zhang, Yuanyuan
Peng, Chaoquan
Source
Cardiology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
MicroRNA-125b (miR-125b) has been recognized as one of the key regulators of the inflammatory responses in cardiovascular diseases recently.
This study sought to dissect the role of miR-125b in modulating the function of endothelial progenitor cells (EPCs) in the inflammatory environment of ischemic hearts.
Methods.
EPCs were cultured and transfected with miR-125b mimic and negative control mimic.
Cell migration and adhesion assays were performed after tumor necrosis factor-α (TNF-α) treatment to determine EPC function.
Cell apoptosis was analyzed by flow cytometry.
The activation of the NF-κB pathway was measured by western blotting.
EPC-mediated neovascularization in vivo was studied by using a myocardial infarction model.
Results.
miR-125b-overexpressed EPCs displayed improved cell migration, adhesion abilities, and reduced cell apoptosis compared with those of the NC group after TNF-α treatment.
miR-125b overexpression in EPCs ameliorated TNF-α-induced activation of the NF-κB pathway.
Mice transplanted with miR-125b-overexpressed EPCs showed improved cardiac function recovery and capillary vessel density than the ones transplanted with NC EPCs.
Conclusions.
miR-125b protects EPCs against TNF-α-induced inflammation and cell apoptosis by attenuating the activation of NF-κB pathway and consequently improves the cardiac function recovery and EPC-mediated neovascularization in the ischemic hearts.
American Psychological Association (APA)
Yang, Ke& Liu, Xing& Lin, Wanwen& Zhang, Yuanyuan& Peng, Chaoquan. 2020. Upregulation of MicroRNA-125b Leads to the Resistance to Inflammatory Injury in Endothelial Progenitor Cells. Cardiology Research and Practice،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1152518
Modern Language Association (MLA)
Yang, Ke…[et al.]. Upregulation of MicroRNA-125b Leads to the Resistance to Inflammatory Injury in Endothelial Progenitor Cells. Cardiology Research and Practice No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1152518
American Medical Association (AMA)
Yang, Ke& Liu, Xing& Lin, Wanwen& Zhang, Yuanyuan& Peng, Chaoquan. Upregulation of MicroRNA-125b Leads to the Resistance to Inflammatory Injury in Endothelial Progenitor Cells. Cardiology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1152518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152518