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Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse
Joint Authors
Saika, Shizuya
Yamada, Hiroshi
Yoshida, Munehito
Kitano, Takashi
Kida, Maki
Okada, Yuka
Source
Dermatology Research and Practice
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
We investigated the effects of loss of inducible nitric oxide synthase (iNOS) on the healing process of cutaneous excisional injury by using iNOS-null (KO) mice.
Population of granulation tissue-related cell types, that is, myofibroblasts and macrophages, growth factor expression, and reepithelialization were evaluated.
Methods.
KO and wild type (WT) mice of C57BL/6 background were used.
Under general anesthesia two round full-thickness excision wounds of 5.0 mm in diameter were produced in dorsal skin.
After specific intervals of healing, macroscopic observation, histology, immunohistochemistry, and real-time reverse transcription-polymerase chain reaction (RT-PCR) were employed to evaluate the healing process.
Results.
The loss of iNOS retards granulation tissue formation and reepithelialization in excision wound model in mice.
Detailed analyses showed that myofibroblast appearance, macrophage infiltration, and mRNA expression of transforming growth factor b and of collagen 1α2 were all suppressed by lacking iNOS.
Conclusions.
iNOS is required in the process of cutaneous wound healing.
Lacking iNOS retards macrophage invasion and its expression of fibrogenic components that might further impair fibrogenic behaviors of fibroblasts.
American Psychological Association (APA)
Kitano, Takashi& Yamada, Hiroshi& Kida, Maki& Okada, Yuka& Saika, Shizuya& Yoshida, Munehito. 2017. Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse. Dermatology Research and Practice،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152656
Modern Language Association (MLA)
Kitano, Takashi…[et al.]. Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse. Dermatology Research and Practice No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1152656
American Medical Association (AMA)
Kitano, Takashi& Yamada, Hiroshi& Kida, Maki& Okada, Yuka& Saika, Shizuya& Yoshida, Munehito. Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse. Dermatology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152656
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152656