Maoto, a Traditional Japanese Herbal Medicine, Inhibits Uncoating of Influenza Virus
Joint Authors
Masui, Shinta
Nabeshima, Shigeki
Ajisaka, Kazuhiko
Yamauchi, Kei
Itoh, Ryota
Ishii, Kazunari
Soejima, Toshinori
Hiromatsu, Kenji
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We previously reported in randomized controlled trials that maoto, a traditional herbal medicine, showed clinical and virological efficacy for seasonal influenza.
In this study, a culturing system for influenza was used to test the effect of maoto.
A549 cells in the culture were infected with influenza virus A (PR8) and followed after treatment with maoto; the virus titers in the culture supernatant, intracellular viral proteins, and viral RNA were determined.
When infected cells were cultured with maoto for 24 hr, the virus titer and protein were significantly reduced compared with medium only.
Other subtypes, A/H3N2, H1N1pdm, and B, were also inhibited by maoto.
Proliferation of viral RNA in a 6 hr culture was inhibited by maoto in the early phase, especially in the first 30 min.
Focusing on the entry step of the influenza virus, we found that endosomal pH, regulated by vacuolar-type H+ ATPase (V-ATPase) located in the membrane, was increased when treated with maoto.
We also found that uncoating of influenza viruses was also inhibited by maoto, resulting in the increase of the number of virus particles in endosomes.
These results strongly suggest that the inhibition of endosomal acidification by maoto results in blocking influenza virus entry to cytoplasm, probably through the inhibition of V-ATPase.
The present study provides evidence that supports the clinical use of maoto for the treatment of influenza.
American Psychological Association (APA)
Masui, Shinta& Nabeshima, Shigeki& Ajisaka, Kazuhiko& Yamauchi, Kei& Itoh, Ryota& Ishii, Kazunari…[et al.]. 2017. Maoto, a Traditional Japanese Herbal Medicine, Inhibits Uncoating of Influenza Virus. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1152937
Modern Language Association (MLA)
Masui, Shinta…[et al.]. Maoto, a Traditional Japanese Herbal Medicine, Inhibits Uncoating of Influenza Virus. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1152937
American Medical Association (AMA)
Masui, Shinta& Nabeshima, Shigeki& Ajisaka, Kazuhiko& Yamauchi, Kei& Itoh, Ryota& Ishii, Kazunari…[et al.]. Maoto, a Traditional Japanese Herbal Medicine, Inhibits Uncoating of Influenza Virus. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1152937
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152937