iTRAQ-Based Proteomics of Chronic Renal Failure Rats after FuShengong Decoction Treatment Reveals Haptoglobin and Alpha-1-Antitrypsin as Potential Biomarkers
Joint Authors
Yang, Yu
Wei, Junmeng
Huang, Xuekuan
Wu, Mingjun
Lv, Zhenbing
Tong, Pan
Chang, Rui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Chronic renal failure (CRF) has become a global health problem and bears a huge economic burden.
FuShengong Decoction (FSGD) as traditional Chinese medicine has multiple pharmacological effects.
Objectives.
To understand the underlying molecular mechanism and signaling pathway involved in the FSGD treatment of CRF and screen differentially expressed proteins in rats with CRF treated with FSGD.
Methods.
Thirty-three male Sprague-Dawley rats were randomly divided into control group, CRF group, and FSGD group.
Differentially expressed proteins were screened by iTRAQ coupled with nanoLC-MS/MS, and these identified proteins were later analyzed by GO, KEGG, and STRING.
Additionally, haptoglobin (HP) and alpha-1-antitrypsin (AAT) were finally verified by ELISA, Western blot, and real time PCR.
Results.
A total of 417 proteins were identified.
Nineteen differentially expressed proteins were identified in the FSGD group compared with the model group, of which 3 proteins were upregulated and 16 proteins were downregulated.
Cluster analysis indicated that inflammatory response was associated with these proteins and complement and coagulation cascade pathways were predominantly involved.
The validation methods further confirmed that the levels of HP and AAT were significantly increased.
Conclusions.
HP and AAT may be the important biomarkers in the pathogenesis of CRF and FSGD therapy.
American Psychological Association (APA)
Yang, Yu& Wei, Junmeng& Huang, Xuekuan& Wu, Mingjun& Lv, Zhenbing& Tong, Pan…[et al.]. 2017. iTRAQ-Based Proteomics of Chronic Renal Failure Rats after FuShengong Decoction Treatment Reveals Haptoglobin and Alpha-1-Antitrypsin as Potential Biomarkers. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1153008
Modern Language Association (MLA)
Yang, Yu…[et al.]. iTRAQ-Based Proteomics of Chronic Renal Failure Rats after FuShengong Decoction Treatment Reveals Haptoglobin and Alpha-1-Antitrypsin as Potential Biomarkers. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1153008
American Medical Association (AMA)
Yang, Yu& Wei, Junmeng& Huang, Xuekuan& Wu, Mingjun& Lv, Zhenbing& Tong, Pan…[et al.]. iTRAQ-Based Proteomics of Chronic Renal Failure Rats after FuShengong Decoction Treatment Reveals Haptoglobin and Alpha-1-Antitrypsin as Potential Biomarkers. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1153008
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153008