Effects of Brucine on the OPGRANKLRANK Signaling Pathway in MDA-MB-231 and MC3T3-E1 Cell Coculture System
Joint Authors
Pei, Xiaohua
Wu, Ruixian
Li, Qian
Hu, Kefei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The present study examined the effects of brucine on the OPG/RANKL/RANK signaling pathway for exploring the mechanism of brucine suppression of bone metastasis in breast cancer.
MDA-MB-231 breast cancer cells and mouse osteoblast MC3T3-E1 cells were cocultured to mimic the breast cancer bone metastasis microenvironment in vitro.
qRT-PCR and Western blotting were used to detect the expressions of OPG and RANKL at the mRNA and protein levels, respectively, in brucine-treated cultures and they were compared to those in untreated cultures.
We aimed to understand the effect of brucine on the entire OPG/RANKL/RANK signaling pathway after analyzing these effects.
Results showed that brucine treatment significantly increased both the OPG mRNA/RANKL mRNA expression ratio and the OPG protein/RANKL protein ratio in cocultures compared to those in untreated cocultures (P<0.01).
Brucine, therefore, plays a regulatory role in the OPG/RANKL/RANK signaling pathway, suggesting that it can indirectly control osteoclasts by regulating the expression and secretion of OPG and RANKL in osteoblast cells, thereby inhibiting the differentiation and bone resorption function of osteoclasts.
American Psychological Association (APA)
Wu, Ruixian& Li, Qian& Pei, Xiaohua& Hu, Kefei. 2017. Effects of Brucine on the OPGRANKLRANK Signaling Pathway in MDA-MB-231 and MC3T3-E1 Cell Coculture System. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1153061
Modern Language Association (MLA)
Wu, Ruixian…[et al.]. Effects of Brucine on the OPGRANKLRANK Signaling Pathway in MDA-MB-231 and MC3T3-E1 Cell Coculture System. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1153061
American Medical Association (AMA)
Wu, Ruixian& Li, Qian& Pei, Xiaohua& Hu, Kefei. Effects of Brucine on the OPGRANKLRANK Signaling Pathway in MDA-MB-231 and MC3T3-E1 Cell Coculture System. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1153061
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153061