Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway
Joint Authors
Shao, Zengwu
Deng, Xiangyu
Wu, Wei
Liang, Hang
Huang, Donghua
Jing, Doudou
Zheng, Dong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Purpose.
To explore the effect and possible mechanism of icariin, a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum that was applied to IL-1β pretreated human nucleus pulposus (NP) cells.
Methods.
Human NP cells were isolated from intervertebral discs of patients with scoliosis and lumbar spondylolisthesis.
The cells were divided into five groups: A (blank control); B (20 ng/ml IL-1β); C (20 ng/ml IL-1β + 20 μM icariin); D (20 μM icariin + 20 ng/ml IL-1β + 25 μM LY294002); E (20 ng/ml IL-1β + 25 μM LY294002).
For each of the five groups, the CCK8, apoptosis rates, ROS rates, and JC-1 rates were determined and an electron micrograph was performed.
Different expression levels of apoptosis proteins and proteins in the PI3K/AKT pathway were detected via western blot.
Results.
We found that the damage effects on human nucleus pulposus cells from 20 ng/ml of IL-1β exposure were attenuated by icariin.
When the PI3K/AKT pathway was blocked by LY294002, a specific inhibitor of this pathway, the protective effect of icariin was impaired.
In summary, icariin might be a protective traditional Chinese medicine, which prevents inflammation-induced degeneration of intervertebral discs partly through the PI3K/AKT pathway.
American Psychological Association (APA)
Deng, Xiangyu& Wu, Wei& Liang, Hang& Huang, Donghua& Jing, Doudou& Zheng, Dong…[et al.]. 2017. Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1153234
Modern Language Association (MLA)
Deng, Xiangyu…[et al.]. Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1153234
American Medical Association (AMA)
Deng, Xiangyu& Wu, Wei& Liang, Hang& Huang, Donghua& Jing, Doudou& Zheng, Dong…[et al.]. Icariin Prevents IL-1β-Induced Apoptosis in Human Nucleus Pulposus via the PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1153234
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153234