Tetramethylpyrazine Protects against Early Brain Injury after Experimental Subarachnoid Hemorrhage by Affecting Mitochondrial-Dependent Caspase-3 Apoptotic Pathway
Joint Authors
Ni, Xiaojia
Ye, Zhen-nan
Hang, Chun-hua
Li, Shaoxue
Xiao, Xiaolan
Zhao, Junjie
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study was to test the hypothesis that tetramethylpyrazine (TMP) protected against early brain injury after subarachnoid hemorrhage (SAH) by affecting the mitochondrial-dependent caspase-3 apoptotic pathway.
TMP was administrated after the rats’ prechiasmatic SAH mode.
Animal neurobehavioral functions were assessed and the mitochondrial morphology, mitochondrial and cytoplasmic calcium, and mitochondrial membrane potential changes (Δψm) of the brain tissues were measured.
The expressions of cytoplasmic cytochrome c (cyt c), second mitochondria-derived activator of caspases (Smac), and cleaved caspase-3 B-cell lymphoma 2 (bcl-2) in cells were determined and cellular apoptosis was detected.
The treatment of TMP resulted in less apoptotic cells and milder mitochondrial injury and potentially performed better in the neurobehavioral outcome compared to those with saline.
Also, TMP ameliorated calcium overload in mitochondria and cytoplasm and alleviated the decrease of Δψm.
In addition, TMP inhibited the expression of cytoplasmic cyt c, Smac, and cleaved caspase-3, yet it upregulated the expression of bcl-2.
These findings suggest that TMP exerts an antiapoptosis property in the SAH rat model and this is probably mediated by the caspase-3 apoptotic pathway triggered by mitochondrial calcium overload.
The finding offers a new therapeutic candidate for early brain injury after SAH.
American Psychological Association (APA)
Li, Shaoxue& Xiao, Xiaolan& Ni, Xiaojia& Ye, Zhen-nan& Zhao, Junjie& Hang, Chun-hua. 2017. Tetramethylpyrazine Protects against Early Brain Injury after Experimental Subarachnoid Hemorrhage by Affecting Mitochondrial-Dependent Caspase-3 Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1153518
Modern Language Association (MLA)
Li, Shaoxue…[et al.]. Tetramethylpyrazine Protects against Early Brain Injury after Experimental Subarachnoid Hemorrhage by Affecting Mitochondrial-Dependent Caspase-3 Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1153518
American Medical Association (AMA)
Li, Shaoxue& Xiao, Xiaolan& Ni, Xiaojia& Ye, Zhen-nan& Zhao, Junjie& Hang, Chun-hua. Tetramethylpyrazine Protects against Early Brain Injury after Experimental Subarachnoid Hemorrhage by Affecting Mitochondrial-Dependent Caspase-3 Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1153518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153518