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Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts
Joint Authors
Kulma, Anna
Szopa, Jan
Czemplik, Magdalena
Korzun-Chłopicka, Urszula
Szatkowski, Michał
Działo, Magdalena
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-24
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The goal of this study was to evaluate the most effective technique for extraction of phenolics present in flax shives and to assess their effect on human fibroblasts.
Flax shives are by-products of fibre separation, but they were found to be a rich source of phenolic compounds and thus might have application potential.
It was found that the optimal procedure for extraction of phenolics was hydrolysis enhanced by the ultrasound with NaOH for 24 h at 65°C and subsequent extraction with ethyl acetate.
The influence of the flax shives extract on fibroblast growth and viability was assessed using the MTT and SRB tests.
Moreover, the influence of flax shives extract on the extracellular matrix remodelling process was verified.
The 20% increase of the viability was observed upon flax shives extract treatment and the decrease of mRNA collagen genes, an increase of matrix metalloproteinase gene expression, and reduction in levels of interleukin 6, interleukin 10, and suppressor of cytokinin signaling 1 mRNA were observed.
Alterations in MCP-1 mRNA levels were dependent on flax shives extract concentration.
Thus, we suggested the possible application of flax shives extract in the wound healing process.
American Psychological Association (APA)
Czemplik, Magdalena& Korzun-Chłopicka, Urszula& Szatkowski, Michał& Działo, Magdalena& Szopa, Jan& Kulma, Anna. 2017. Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1153525
Modern Language Association (MLA)
Czemplik, Magdalena…[et al.]. Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1153525
American Medical Association (AMA)
Czemplik, Magdalena& Korzun-Chłopicka, Urszula& Szatkowski, Michał& Działo, Magdalena& Szopa, Jan& Kulma, Anna. Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1153525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153525