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A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes
Joint Authors
Faria, Robson Xavier
Santos, José Augusto Albuquerque
Cavalcante, Viviane Paixão
Rangel, Leonardo da Silva
Leite, João Claudio Vitória Atico
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Schistosomiasis is a disease endemic in several states of Brazil.
The population control of the transmitter mollusks is done with Bayluscide WP 70®, in the control programs.
OMS preconize molluscicidal assays using Becker with 500 mL of final volume, restringing the number of natural products and synthetic drugs to be tested in function of high quantity of material necessary.
A new technique to assess the toxic effects for Biomphalaria sp.
is the purpose of this work, for developing adaptation for this aquatic organism, using a low volume of test solution in 24-well plates.
We used Biomphalaria glabrata (10–12 mm size) in a static system, consisting of the following components: Becker containing 10 snails or 24-well plates where snails were individualized for a volume of 2 mL per well for 24 and 48 hours.
For the assays, we added aqueous solutions of Bayluscide WP 70, at a concentration of 1–5 mg/L, distilled water, and 1% dimethyl sulfoxide.
Data were evaluated using Kappa’s coefficient, Z factor validation, and comparison study.
This technique to assess the toxic effect has proven to be a useful tool to detect lethal and sublethal effects, which could be used as a new evaluation protocol.
American Psychological Association (APA)
Santos, José Augusto Albuquerque& Cavalcante, Viviane Paixão& Rangel, Leonardo da Silva& Leite, João Claudio Vitória Atico& Faria, Robson Xavier. 2017. A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1153552
Modern Language Association (MLA)
Santos, José Augusto Albuquerque…[et al.]. A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1153552
American Medical Association (AMA)
Santos, José Augusto Albuquerque& Cavalcante, Viviane Paixão& Rangel, Leonardo da Silva& Leite, João Claudio Vitória Atico& Faria, Robson Xavier. A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1153552
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153552